Protein extraction and separation in polyacrylamide slab gel electrophoresis with Laemmli system (SDS-PAGE) were used to establish the detection level of protein raw materials in mixtures with porcine meat in boneless cooked ham. Model systems of boneless cooked ham with soy protein isolates, caseinate, skim powdered milk, bovine plasma, porcine plasma and whey proteins were studied. The quantification level of this method was 0.5% for soy protein isolates, caseinate and bovine plasma and 1.0% for porcine plasma, milk powder and whey proteins in boneless cooked ham. The electrophoretic method proved to be useful to identify some proteinic raw materials in porcine meat products and verify compliance with Argentine legislation. It may be used as a control methodology.
[Identification of extrinsic proteins in boneless cooked ham by SDS-PAGE: detection level in model systems] Publishing Authors By Initials
[Identification of extrinsic proteins in boneless cooked ham by SDS-PAGE: detection level in model systems] Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Archivos latinoamericanos de nutrición
VOLUME: 56
Page Numbers: 282-7
Journal Abbreviation:
ISSN: 0004-0622
DAY: 25
MONTH: Sep
YEAR: 2006
[Identification of extrinsic proteins in boneless cooked ham by SDS-PAGE: detection level in model systems] Information
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LANGUAGE: spa
NlmUniqueID: 67507
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Grant and Affiliation Information for [Identification of extrinsic proteins in boneless cooked ham by SDS-PAGE: detection level in model systems]
AFFILIATION: Cátedra de Bromatologia, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Country: Venezuela
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MEDLINETA: Arch Latinoam Nutr
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