Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library.

Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library. Research Abstract Details 

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Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library. Abstract Text:

Dezhong Yin,Yinduo Ji,

Antisense RNA technology has been used effectively to downregulate gene expression in a variety of bacterial systems. Regulated antisense RNA strategy provides an important approach to identify and characterize essential genes critical to bacterial growth in vitro and in vivo. This strategy allows selective genes to be turned on or off and to be expressed at certain levels. The availability of the Staphylococcus aureus (S. aureus) genome sequence makes it feasible to generate a gene-specific antisense RNA library. The combination of regulated antisense RNA technology and the gene-specific antisense RNA library allows for genome-wide analyses of functions of staphylococcal gene products for growth in culture and survival during infection.

Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library. Publishing Authors By Initials

D Yin,Y Ji,

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Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 416

Page Numbers: 297-306

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 21

MONTH: 02

YEAR: 2007

Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library. Information

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LANGUAGE: eng

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Country: United States

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MEDLINETA: Methods Mol Biol

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