Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer.

Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer. Research Abstract Details 

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Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer. Abstract Text:

Joseph F Smith,Saleh Mahmood,Fei Song,Arlene Morrow,Dominic Smiraglia,Xueli Zhang,Ashwani Rajput,Michael J Higgins,Anton Krumm,Nicholas J Petrelli,Joseph F Costello,Hiroki Nagase,Christoph Plass,William A Held,Joseph F Smith,Saleh Mahmood,Fei Song,Arlene Morrow,Dominic Smiraglia,Xueli Zhang,Ashwani Rajput,Michael J Higgins,Anton Krumm,Nicholas J Petrelli,Joseph F Costello,Hiroki Nagase,Christoph Plass,William A Held,

Restriction landmark genomic scanning (RLGS), a method for the two-dimensional display of end-labeled DNA restriction fragments, was utilized to identify genomic regions of CpG island methylation associated with human colon cancer. An average of 1.5% of the RLGS loci/spots are lost or significantly reduced in sporadic primary colon tumors relative to normal colon mucosa from the same patient. This may represent tumor specific methylation of about 400 CpG islands in sporadic colon cancer. A number of RLGS loci exhibiting frequent loss associated with colon cancer were cloned. DNA sequence analysis indicated that the RLGS loci identified genomic regions characteristic of CpG islands. A number of methods including bisulfite genomic sequencing as well as quantitative MassARRAY methylation analysis (www.sequenom.com) confirmed tumor specific methylation at several of these loci. DNA database searches indicated that candidate genes associated with these loci include transcription factors and genes involved in signal transduction (52%), and genes of unknown function (37%). Expression analysis using quantitative real time RT-PCR indicates that methylation of some CpG islands located in non-promoter regions were associated with upregulation of gene expression in colorectal cancer. These results indicate that alterations in methylation status within CpG islands in colon tumors may have complex consequences on gene expression and tumorigenesis, sometimes resulting in up regulation or ectopic gene expression that may involve novel regulatory mechanisms.

Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer. Publishing Authors By Initials

JF Smith,S Mahmood,F Song,A Morrow,D Smiraglia,X Zhang,A Rajput,MJ Higgins,A Krumm,NJ Petrelli,JF Costello,H Nagase,C Plass,WA Held,JF Smith,S Mahmood,F Song,A Morrow,D Smiraglia,X Zhang,A Rajput,MJ Higgins,A Krumm,NJ Petrelli,JF Costello,H Nagase,C Plass,WA Held,

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Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Epigenetics : official journal of the DNA Methylat

VOLUME: 2

Page Numbers: 161-72

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ISSN: 1559-2308

DAY: 27

MONTH: 07

YEAR: 2007

Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer. Information

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LANGUAGE: eng

NlmUniqueID: 101265293

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Grant and Affiliation Information for Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer.

AFFILIATION: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York, USA.

Country: United States

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MEDLINETA: Epigenetics

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