Identification of differentially expressed genes in mouse Pax6 heterozygous lenses.

Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Research Abstract Details 

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Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Abstract Text:

Bharesh K Chauhan,Weiyan Zhang,Kveta Cveklova,Marc Kantorow,Ales Cvekl,

PURPOSE: Pax6 is a critical regulator of the developing lens, other ocular tissues, central nervous system, and pancreas. Downstream targets of Pax6 are largely unknown. The present study was designed to identify differentially expressed genes in Pax6 heterozygous and normal mouse lenses. METHODS: RNAs from 8-week-old normal and Pax6 heterozygous mouse lenses were analyzed by both RT-PCR differential display and a candidate-gene approach. The expression levels of identified genes were confirmed by semiquantitative RT-PCR. RESULTS: Eight transcripts encoding phosphatase inhibitor Pip-1 protein, heat shock protein Hsp40, Purkinje cell protein Pcp4, an expressed sequence tag (EST; AA331381) originally reported in a brain-specific library, Pitx3, and CBP, were confirmed to be downregulated in the Pax6 heterozygous mouse lenses. alphaB- and betaA3/A1-crystallin transcripts exhibited decreased expression in Pax6 heterozygous lenses, whereas the expression levels of other crystallins were virtually unchanged. CONCLUSIONS: The present data identify eight genes with expression levels that are decreased in Pax6 heterozygous lenses and provide evidence that four functional categories of transcripts-namely, small hsps (alphaB-crystallin and Hsp40), crystallins (alphaB- and betaA3/A1-crystallin), transcription factors (Pitx-3 and CBP), and components of signal transduction cascades (Pip-1) are under direct or indirect transcriptional control by Pax6.

Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Publishing Authors By Initials

BK Chauhan,W Zhang,K Cveklova,M Kantorow,A Cvekl,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Investigative ophthalmology & visual science

VOLUME: 43

Page Numbers: 1884-90

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 26

MONTH: Jun

YEAR: 2002

Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Information

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LANGUAGE: eng

NlmUniqueID: 7703701

Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for Identification of differentially expressed genes in mouse Pax6 heterozygous lenses.

AFFILIATION: Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Country: United States

AGENCY: United States NEI

GRANT: R01 EY013022-02

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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