Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4.

Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Research Abstract Details 

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Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Abstract Text:

Ruxian Lin,Yuning Sun,Chunrong Li,Chunhua Xie,Shengqi Wang,

To identify candidate genes in response to ionizing radiation (IR) and discover new targets for basic research and radiation protection, whole human genome bioarrays were used to examine gene expression profiles in human lymphoblastoid AHH-1 cells exposed to IR. The results were confirmed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). In addition, the effects of ionizing radiation on cell growth, cell cycles and apoptosis were also examined. The microarray analysis revealed a set of IR responsive genes, including 906 genes at 4 hours and 789 genes at 24 hours after exposure to 5 Gy IR. The processes of cell cycles, apoptosis, signal transduction, and DNA repair involved a high percentage of IR responsive genes, among which, caspase-4 was most strongly induced by irradiation. Consistent with this, downregulation of caspase-4 expression by antisense oligonucleotides significantly increased cell viability and protected cells from undergoing apoptosis induced by IR. Taken together, the results suggested that caspase-4 plays an important role in radiation-induced apoptosis.

Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Publishing Authors By Initials

R Lin,Y Sun,C Li,C Xie,S Wang,

For similar nucleic acids, nucleotides, and nucleosides: antisense elements (genetics): oligonucleotides, antisense research abstracts see: nucleic acids, nucleotides, and nucleosides: antisense elements (genetics): oligonucleotides, antisense research

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Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Oligonucleotides

VOLUME: 17

Page Numbers: 314-26

Journal Abbreviation:

ISSN: 1545-4576

DAY: 26

MONTH: 11

YEAR: 2007

Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Information

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LANGUAGE: eng

NlmUniqueID: 101188415

Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Keywords Mesh Terms:

KEYWORDS: Oligonucleotides, Antisense

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4. Information

Substance Name: Caspases, Initiator

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Identification of differentially expressed genes in human lymphoblastoid cells exposed to irradiation and suppression of radiation-induced apoptosis with antisense oligonucleotides against caspase-4.

AFFILIATION: Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China.

Country: United States

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MEDLINETA: Oligonucleotides

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