[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization]

[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization] Research Abstract Details 

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[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization] Abstract Text:

Jie Chen,Shu-yun Zhou,Ru Feng,

OBJECTIVE: To understand the differential gene expression profiles between bone marrow cells and mobilized peripheral blood CD34(+) cells. METHODS: Suppression subtractive hybridization (SSH) was employed to identify the genes differentially expressed in bone marrow and mobilized peripheral blood CD34(+) cells obtained from a healthy donor. RESULTS: Twenty-one differentially expressed genes were identified that could be categorized into S-phase- or G(2)-M cell cycle-related genes and CCAAT/enhancer binding protein (C/EBP) transcription factor family. The genes highly expressed in bone marrow CD34(+) cells indicated low expression of their counterparts as SSH demands strict paired comparison between the 2 types of CD34(+) cells of different origins. CONCLUSION: CD34(+) cells derived from bone marrow are featured by more active proliferation than are those from mobilized peripheral blood.

[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization] Publishing Authors By Initials

J Chen,SY Zhou,R Feng,

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[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Di 1 jun yi da xue xue bao = Academic journal of t

VOLUME: 22

Page Numbers: 823-5

Journal Abbreviation: Di Yi Jun Yi Da Xue Xue Bao

ISSN: 1000-2588

DAY: 25

MONTH: Sep

YEAR: 2002

[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization] Information

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LANGUAGE: chi

NlmUniqueID: 9426110

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AFFILIATION: Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. chinsyou@263.net

Country: China

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MEDLINETA: Di Yi Jun Yi Da Xue Xue Bao

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