Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR.

Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Abstract Text:

Lingwei Ruan,Tian Luo,Fang Li,Xun Xu,Lingwei Ruan,Tian Luo,Fang Li,Xun Xu,

Most research on the adaptation of thermophiles is focused on their adaptation to heat stress; only a few studies are focused on their cold adaptation. In this report, the thermophilic bacterium Rhodothermus sp. XMH10 was examined to gain a better understanding of gene expression in response to low temperature. Random arbitrarily primed polymerase chain reaction (RAP-PCR) was used to isolate and identify differentially expressed genes of bacteria grown at 45 degrees C (lowest) compared to those at 75 degrees C (optimal). Fifty-three differential cDNA fragments in total were isolated. Among them, 35 different cDNAs were analyzed by Northern blot, and 17 were confirmed to be differentially expressed at the transcriptional levels. These genes reflected a profile of differential expression and were involved in many physiological processes such as metabolism, cell membrane alterations, and regulatory adaptive response; most of them have never been previously reported. This study provides some new information on the adaptation of thermophilic bacteria to environmental temperature stress.

Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Publishing Authors By Initials

L Ruan,T Luo,F Li,X Xu,L Ruan,T Luo,F Li,X Xu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current microbiology

VOLUME: 55

Page Numbers: 543-8

Journal Abbreviation: Curr. Microbiol.

ISSN: 0343-8651

DAY: 5

MONTH: 10

YEAR: 2007

Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7808448

Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR.

AFFILIATION: School of Life Sciences, Xiamen University, Xiamen, People's Republic of China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Curr Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of differentially expressed genes from Rhodothermus sp XMH10 in response to low temperature using random arbitrarily primed PCR Related Publications

• Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
• Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography.
• Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat.
• [Analysis of the mutations of BRCA1 in 9 familiar breast cancer patients]
• [Application of the interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure]
• [Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB]
• [Changes in the activity of platelet L-arginine/nitric oxide pathway in hypercholesterolemia patients]
• [Clinical significance of changes in insulin resistance and secretion in multiple organ dysfunction syndrome patients complicated by trauma and hemorrhage]
• [Cloning and sequencing of the genes coding for the histidine-rich protein II of Plasmodium falciparum]
• [Contrast agents in magnetic resonance imaging: development and problems]
• [Differentially expressed genes associated with cold acclimation]
• [Effects of embryonic neural stem cells and glial cell line-derived neurotrophic factor in the repair of spinal cord injury]
• [Effects of ulinastatin on interleukin-8 during one-lung ventilation in surgery]
• [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]
• [Mechanism of modulation of synaptic efficacy by stress and learning]
• [Opinion on hydatidosis control by immuno-prophylaxis]
• [Oral mucosa graft combined with a local turn-over flap for urethral reconstruction in various hypospadias]
• [Pharmacognostical studies on the roots of Euphorbia prolifera]
• [Pharmacological comparison between virosecurinine and securinine]
• [Phenotyping and genotyping studies in a family with the compound heterozygosity for a deletional delta beta-thalassemia and a beta-thalassemia]
• [Properties of whole-cell potassium currents in mechanically dissociated Drosophila larval central neurons]
• [Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot]
• [Retrospective analysis of effectiveness of tonsillar squamous cell carcinoma: report of 108 cases]
• [Significance of hRad17 mRNA expression in human gastric cancer]
• [Study of functional L1 retrotransposon in human type 2 diabetes susceptibility loci]
• [Study on diarrhea disease and Escherichia coli strains harboring HPI pathogenicity island of Yersinia enterolitica in Shandong province]
• [The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice]
• [The influence of postburn administration of rhGH on the host inflammatory response]
• [The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery]
• [Tissue-specific expression of Na(+)-H(+) exchanger isoforms at two developmental stages of human fetus]