Identification of CYP2C9*2 Allele in HepG2 Cell Line.

Identification of CYP2C9*2 Allele in HepG2 Cell Line. Research Abstract Details 

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Identification of CYP2C9*2 Allele in HepG2 Cell Line. Abstract Text:

Jiezhong Chen,Kenneth Raymond,

Hepatoma is caused by many factors including alcohol, chemicals, viral infection, and chronic inflammation. Cytochrome P450 polymorphism plays an important role in its pathogenesis. CYP2D6, CYP2E1, and CYP1A1 have been identified to be related with hepatic carcinogenesis and tumor size and stage. However, no studies have been performed on CYP2C9, a major CYP in the liver and hepatoma.Aim of the studyTo identify if there is polymorphism of CYP2C9 in a HepG2 cell line.MethodsA pair of primers was used to clone CYP2C9 exon 3 region and subsequently sequenced. The sequence was compared to normal CYP2C9 for identification of any mutation.ResultsA point mutation was identified. It was located in the amino acid number 144 of CYP2C9 protein with the change of normal amino acid arginine into cysteine, which is the same as identified in poor metabolism patients as homozygous CYP2C9*2.ConclusionsThere is a mutation (CYP2C9*2/ CYP2C9*2) in a HepG2 cell line. Thus, polymorphism of CYP2C9 may also be involved in the carcinogenesis of hepatoma as CYPs2D6 and 2E1.

Identification of CYP2C9*2 Allele in HepG2 Cell Line. Publishing Authors By Initials

J Chen,K Raymond,

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Identification of CYP2C9*2 Allele in HepG2 Cell Line. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: International journal of gastrointestinal cancer

VOLUME: 37

Page Numbers: 79-83

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ISSN: 1537-3649

DAY: 10

MONTH: 09

YEAR: 2006

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LANGUAGE: eng

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AFFILIATION: School of Pharmacy and Applied Science, Faculty of Science, Technology and Engineering, La Trobe University, Bendigo, Vic, 3550, Australia.

Country: United States

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MEDLINETA: Int J Gastrointest Cancer

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