Identification of cross-linked peptides from large sequence databases.

Identification of cross-linked peptides from large sequence databases. Research Abstract Details 

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Identification of cross-linked peptides from large sequence databases. Abstract Text:

Oliver Rinner,Jan Seebacher,Thomas Walzthoeni,Lukas Mueller,Martin Beck,Alexander Schmidt,Markus Mueller,Ruedi Aebersold,Oliver Rinner,Jan Seebacher,Thomas Walzthoeni,Lukas Mueller,Martin Beck,Alexander Schmidt,Markus Mueller,Ruedi Aebersold,

We describe a method to identify cross-linked peptides from complex samples and large protein sequence databases by combining isotopically tagged cross-linkers, chromatographic enrichment, targeted proteomics and a new search engine called xQuest. This software reduces the search space by an upstream candidate-peptide search before the recombination step. We showed that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search.

Identification of cross-linked peptides from large sequence databases. Publishing Authors By Initials

O Rinner,J Seebacher,T Walzthoeni,L Mueller,M Beck,A Schmidt,M Mueller,R Aebersold,O Rinner,J Seebacher,T Walzthoeni,L Mueller,M Beck,A Schmidt,M Mueller,R Aebersold,

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Identification of cross-linked peptides from large sequence databases. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nature methods

VOLUME: 5

Page Numbers: 315-8

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ISSN: 1548-7105

DAY: 9

MONTH: 03

YEAR: 2008

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LANGUAGE: eng

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MEDLINETA: Nat Methods

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