Identification of both calpains I and II in nucleated chicken erythrocytes.

Identification of both calpains I and II in nucleated chicken erythrocytes. Research Abstract Details 

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Identification of both calpains I and II in nucleated chicken erythrocytes. Abstract Text:

T Murakami,M Ueda,T Hamakubo,T Murachi,

Chicken erythrocytes were found to contain two species of calpains which differ in elution profile from DEAE-cellulose and in Ca2+ requirement. After partial purification, one of them was half-maximally activated by 10 microM Ca2+ and the other by 180 microM Ca2+. The low- and high-Ca2+-requiring proteases cross-reacted only with the respective monospecific antibodies for mammalian calpain I and calpain II, respectively. Approximately 5 times more calpain I than calpain II is present in chicken erythrocytes. By immunoelectrophoretic blot analysis, both calpains I and II from chicken erythrocytes were proved to be heterodimers composed of 76 and 28 kDa, and 80 and 28 kDa subunits, respectively. Our present finding that the heavy subunit of calpain I is smaller than that of calpain II is noteworthy, since the opposite is known to be true of various mammalian calpains. An immunological study has revealed that the calpain I newly found in chicken erythrocytes is not derived from calpain II. Thus, the co-existence of calpains I and II in one animal species also holds in chickens, contrary to the previously advocated notion that chickens have only one type of calpain.

Identification of both calpains I and II in nucleated chicken erythrocytes. Publishing Authors By Initials

T Murakami,M Ueda,T Hamakubo,T Murachi,

For similar natural sciences: chemistry: chemistry, physical: molecular weight research abstracts see: natural sciences: chemistry: chemistry, physical: molecular weight research

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Identification of both calpains I and II in nucleated chicken erythrocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 168-71

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1988

Identification of both calpains I and II in nucleated chicken erythrocytes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Identification of both calpains I and II in nucleated chicken erythrocytes. Keywords Mesh Terms:

KEYWORDS: Molecular Weight

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Identification of both calpains I and II in nucleated chicken erythrocytes. Information

Substance Name: Calpain

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Identification of both calpains I and II in nucleated chicken erythrocytes.

AFFILIATION: Department of Home Economics, Kyoto Bunkyo Junior College.

Country: JAPAN

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MEDLINETA: J Biochem

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