Identification of an osteogenic protein-1 responsive element in the aggrecan promoter.

Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Research Abstract Details 

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Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Abstract Text:

Lee-Chuan C Yeh,John C Lee,

Previous studies have demonstrated that osteogenic protein-1 (OP-1), a member of the bone morphogenetic protein (BMP) subfamily of the TGF-beta superfamily, stimulates aggrecan synthesis. To investigate transcriptional regulation of the aggrecan gene by OP-1, we constructed a clone containing a 1 kb region of the 5'-upstream sequence of the mouse aggrecan gene fused to the promoter-less luciferase reporter gene in pGL2-Basic vector. A series of promoter deletion constructs were also generated. Studies of the promoter activity of these DNA constructs in transient-transfected cells revealed that deletion of a 33 bp region rendered the promoter unresponsive to OP-1, BMP-6, and CDMP-1 without affecting BMP-2 and TGF-beta1 responsiveness. Thus, the expression of the mouse aggrecan gene in response to BMPs appears to be the result of a unique combination of different cis-acting elements.

Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Publishing Authors By Initials

LC Yeh,JC Lee,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta1 research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta1 research

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Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 323

Page Numbers: 223-8

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 8

MONTH: Oct

YEAR: 2004

Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Information

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LANGUAGE: eng

NlmUniqueID: 372516

Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta1

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification of an osteogenic protein-1 responsive element in the aggrecan promoter. Information

Substance Name: bone morphogenetic protein 7

Registry Number: 0

Grant and Affiliation Information for Identification of an osteogenic protein-1 responsive element in the aggrecan promoter.

AFFILIATION: Department of Biochemistry, The University of Texas Health Science Center, San Antonio, TX, USA. carolyeh@biochem.uthscsa.edu

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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