Identification of aggrecanase activity in medium of cartilage culture.

Identification of aggrecanase activity in medium of cartilage culture. Research Abstract Details 

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Identification of aggrecanase activity in medium of cartilage culture. Abstract Text:

K Sugimoto,M Takahashi,Y Yamamoto,K Shimada,K Tanzawa,

Erosion of cartilage is a major feature of joint diseases, i.e., osteoarthritis and rheumatoid arthritis, which leads with time to a loss of joint function. Proteolytic cleavage of the aggrecan core protein is a key event in the progress of these joint diseases. Aggrecan degradation has been believed to be mediated by a putative proteinase, aggrecanase. We identified aggrecanase activity in conditioned medium from explant culture of bovine nasal cartilage stimulated by retinoic acid. The activity was partially purified more than 10,000-fold. The enzyme cleaves at the aggrecanase site (Glu(373)-Ala(374)) but not at the MMP site (Asn(341)-Phe(342)) in the interglobular domain of the aggrecan. It also cleaves at Glu(1971)-Leu(1972), which is located in the gap region in the chondroitin sulfate attachment region prior to the aggrecanase site. The enzyme is a typical Ca(2+)-dependent metalloproteinase with a unique salt-dependency and is inhibited by several hydroxamate-based inhibitors for matrix metalloproteinases. Heparin and chondroitin sulfate inhibited the enzyme in a dose-dependent manner, suggesting that the large carbohydorate in aggrecan is important for substrate recognition by aggrecanase.

Identification of aggrecanase activity in medium of cartilage culture. Publishing Authors By Initials

K Sugimoto,M Takahashi,Y Yamamoto,K Shimada,K Tanzawa,

For similar chlorides: sodium chloride research abstracts see: chlorides: sodium chloride research

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Identification of aggrecanase activity in medium of cartilage culture. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 126

Page Numbers: 449-55

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1999

Identification of aggrecanase activity in medium of cartilage culture. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Identification of aggrecanase activity in medium of cartilage culture. Keywords Mesh Terms:

KEYWORDS: Sodium Chloride

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Identification of aggrecanase activity in medium of cartilage culture. Information

Substance Name: aggrecanase

Registry Number: EC 3.4.99.-

Grant and Affiliation Information for Identification of aggrecanase activity in medium of cartilage culture.

AFFILIATION: Biological Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo, 140-8710, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

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