Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis.

Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Research Abstract Details 

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Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Abstract Text:

Agoston Jerga,Ying-Jie Lu,Gustavo E Schujman,Diego de Mendoza,Charles O Rock,

Diacylglycerol kinases (DagKs) are key enzymes in lipid metabolism that function to reintroduce diacylglycerol formed from the hydrolysis of phospholipids into the biosynthetic pathway. Bacillus subtilis is a prototypical Gram-positive bacterium with a lipoteichoic acid structure containing repeating units of sn-glycerol-1-P groups derived from phosphatidylglycerol head groups. The B. subtilis homolog of the prokaryotic DagK gene family (dgkA; Pfam01219) was not a DagK but rather was an undecaprenol kinase. The three members of the soluble DagK protein family (Pfam00781) in B. subtilis were tested by complementation of an E. coli dgkA mutant, and only the essential yerQ gene possessed DagK activity. This gene was dubbed dgkB, and the soluble protein product was purified, and its DagK activity was verified in vitro. Conditional inactivation of dgkB led to the accumulation of diacylglycerol and the cessation of lipoteichoic acid formation in B. subtilis. This study identifies a soluble protein encoded by the dgkB (yerQ) gene as an essential kinase in the diacylglycerol cycle that drives lipoteichoic acid production.

Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Publishing Authors By Initials

A Jerga,YJ Lu,GE Schujman,D de Mendoza,CO Rock,

For similar carbohydrates: glycosides: teichoic acids research abstracts see: carbohydrates: glycosides: teichoic acids research

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Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 21738-45

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 28

MONTH: 05

YEAR: 2007

Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Keywords Mesh Terms:

KEYWORDS: Teichoic Acids

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. Information

Substance Name: Diacylglycerol Kinase

Registry Number: EC 2.7.1.107

Grant and Affiliation Information for Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis.

AFFILIATION: Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105-2794, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM34496

ACRONYM: GM

MEDLINETA: J Biol Chem

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