Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons.

Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Research Abstract Details 

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Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Abstract Text:

Fei Zhou,Daniel Karcher,Ralph Bock,Fei Zhou,Daniel Karcher,Ralph Bock,

Most plastid genes are part of operons and expressed as polycistronic mRNAs. Many primary polycistronic transcripts undergo post-transcriptional processing in monocistronic or oligocistronic units. At least some polycistronic transcripts are not translatable, and endonucleolytic processing may therefore be a prerequisite for translation to occur. As the requirements for intercistronic mRNA processing into stable monocistronic transcript are not well understood, we have sought to define minimum sequence elements that trigger processing and thus are capable of generating stable translatable monocistronic mRNAs. We describe here the in vivo identification of a small intercistronic expression element that mediates intercistronic cleavage into stable monocistronic transcripts. Separation of foreign genes by this element facilitates transgene stacking in operons, and thus will help to expand the range of applications of transplastomic technology.

Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Publishing Authors By Initials

F Zhou,D Karcher,R Bock,F Zhou,D Karcher,R Bock,

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Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Plant journal : for cell and molecular biology

VOLUME: 52

Page Numbers: 961-72

Journal Abbreviation: Plant J.

ISSN: 0960-7412

DAY: 6

MONTH: 09

YEAR: 2007

Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Information

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LANGUAGE: eng

NlmUniqueID: 9207397

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Grant and Affiliation Information for Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons.

AFFILIATION: Max-Planck-Institut für Molekulare Pflanzenphysiologie (MPI-MP), Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany.

Country: England

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MEDLINETA: Plant J

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