Identification of a novel isoform of microsomal triglyceride transfer protein.

Identification of a novel isoform of microsomal triglyceride transfer protein. Research Abstract Details 

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Identification of a novel isoform of microsomal triglyceride transfer protein. Abstract Text:

Peter J Mohler,Mei-Ying Zhu,Anna M Blade,Amy-Joan L Ham,Gregory S Shelness,Larry L Swift,

Microsomal triglyceride transfer protein (MTP) has been studied extensively, primarily because of its role in the assembly of very low density lipoproteins by the liver and chylomicrons by the intestine. Recent studies have suggested that MTP may also play key roles in other cellular processes. In this paper we report the identification of a novel splice variant of MTP in mice. This isoform, MTP-B, has a unique first exon located approximately 2.7 kilobases upstream of canonical MTP (MTP-A) exon 1. The alternative exon encodes 35 amino acids compared with 20 amino acids encoded by exon 1 of MTP-A. MTP-B represents approximately 90% of total MTP mRNA in mouse adipocytes and 3T3-L1 cells and <5% in mouse liver and intestine. Expression of the alternate isoform in mouse liver was confirmed by mass spectrometry. Co-transfection of COS cells with truncated forms of apoB and either MTP-A or MTP-B demonstrated that both isoforms are effective in the assembly and secretion of nascent apoB-containing lipoproteins. Confocal microscopy of 3T3-L1 cells transfected with enhanced green fluorescent protein or DsRed fusions of the two proteins revealed that MTP-A is localized to the endoplasmic reticulum, whereas MTP-B localizes primarily to the Golgi complex in these cells. We conclude that MTP-B functions similarly to MTP-A in lipoprotein assembly. However, in nonlipoprotein-secreting cells, such as the adipocyte, MTP-B may have different localization properties, perhaps reflecting a distinct role in lipid storage and mobilization.

Identification of a novel isoform of microsomal triglyceride transfer protein. Publishing Authors By Initials

PJ Mohler,MY Zhu,AM Blade,AJ Ham,GS Shelness,LL Swift,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Identification of a novel isoform of microsomal triglyceride transfer protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 26981-8

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 16

MONTH: 07

YEAR: 2007

Identification of a novel isoform of microsomal triglyceride transfer protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Identification of a novel isoform of microsomal triglyceride transfer protein. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: physiology

Chemical & Substance for Abstract: Identification of a novel isoform of microsomal triglyceride transfer protein. Information

Substance Name: microsomal triglyceride transfer protein

Registry Number: 0

Grant and Affiliation Information for Identification of a novel isoform of microsomal triglyceride transfer protein.

AFFILIATION: Department of Internal Medicine, University of Iowa School of Medicine, Iowa City, IA 52242, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 57984

ACRONYM: HL

MEDLINETA: J Biol Chem

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