Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli.

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Abstract Text:

C Ulmke,J W Lengeler,K Schmid,

The conjugative plasmid pRSD2 carries a raf operon that encodes a peripheral raffinose metabolic pathway in enterobacteria. In addition to the previously known raf genes, we identified another gene, rafY, which in Escherichia coli codes for an outer membrane protein (molecular mass, 53 kDa) similar in function to the known glycoporins LamB (maltoporin) and ScrY (sucrose porin). Sequence comparisons with LamB and ScrY revealed no significant similarities; however, both lamB and scrY mutants are functionally complemented by RafY. Expressed from the tac promoter, RafY significantly increases the uptake rates for maltose, sucrose, and raffinose at low substrate concentrations; in particular it shifts the apparent K(m) for raffinose transport from 2 mM to 130 microM. Moreover, RafY permits diffusion of the tetrasaccharide stachyose and of maltodextrins up to maltoheptaose through the outer membrane of E. coli. A comparison of all three glycoporins in regard to their substrate selectivity revealed that both ScrY and RafY have a broad substrate range which includes alpha-galactosides while LamB seems to be restricted to malto-oligosaccharides. It supports growth only on maltodextrins but not, like the others, on raffinose and stachyose.

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Publishing Authors By Initials

C Ulmke,JW Lengeler,K Schmid,

For similar proteins: carrier proteins: membrane transport proteins: ion pumps: symporters research abstracts see: proteins: carrier proteins: membrane transport proteins: ion pumps: symporters research

PUBMED ID PMID:

MEDLINE DATE:

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 179

Page Numbers: 5783-8

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 27

MONTH: Sep

YEAR: 1997

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Symporters

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli. Information

Substance Name: lactose permease

Registry Number: 9068-45-5

Grant and Affiliation Information for Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli.

AFFILIATION: Arbeitsgruppe Genetik, Fachbereich Biologie/Chemie, Universität Osnabrück, Germany.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli Related Publications

• A conformational unfolding reaction activates phage fd for the infection of Escherichia coli.
• Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent-tolerant Pseudomonas sp. strain VLB120DeltaC.
• Cerebral near-infrared spectroscopy in adult patients after cardiac surgery is not useful for monitoring absolute values but may reflect trends in venous oxygenation under clinical conditions.
• Characterization of the C-terminal ER membrane anchor of PTP1B.
• Characterization of the genotoxic potential of formaldehyde in V79 cells.
• Clinical observations of sertindole in 53 hospitalised patients with psychotic disorders.
• Clinical validation of a digital transcutaneous PCO2/SpO2 ear sensor in adult patients after cardiac surgery.
• Coarse-grained lattice model for investigating the role of cooperativity in molecular recognition.
• Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method.
• Disorder-induced static antiferromagnetism in cuprate superconductors.
• Emerging drugs for biliary cancer.
• Emphysema and secondary pneumothorax in young adults smoking cannabis.
• Examples of Cross-Section Research on the Effect of the Built Environment on Physical Activity in Diverse Urban Communities: 246: 4:10 PM-4:35 PM.
• Groups of adjacent contour segments for object detection.
• Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli.
• Impact of the SMAS on Frey's syndrome after parotid surgery: a prospective, long-term study.
• Influence of comorbidity on outcome after pulmonary resection in the elderly.
• Inhibition of farnesyl protein transferase sensitizes human MCF-7 breast cancer cells to roscovitine-mediated cell cycle arrest.
• Interplay Between the p53 Tumor Suppressor Protein Family and Cdk5: Novel Therapeutic Approaches for the Treatment of Neurodegenerative Diseases Using Selective Cdk Inhibitors.
• Microbial biofilms: new catalysts for maximizing productivity of long-term biotransformations.
• New options in insulin therapy.
• Optimization of the gbeta1 domain by computational design and by in vitro evolution: structural and energetic basis of stabilization.
• Pediatric pet: indications and value of multimodal imaging.
• SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis.
• Screening and purification for novel cytochrome b5 from uncultured environmental micro-organisms.
• Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity.
• The dynamin middle domain is critical for tetramerization and higher-order self-assembly.
• [Cardiovascular impact of the autonomic neuropathy of diabetes mellitus]
• [On urinary tract infections after a single catheterization]
• [Suddenly occurring exercise intolerance]