Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit.

Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Research Abstract Details 

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Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Abstract Text:

Ann E Tollefson,Baoling Ying,Konstantin Doronin,Peter D Sidor,William S M Wold,Ann E Tollefson,Baoling Ying,Konstantin Doronin,Peter D Sidor,William S M Wold,

A short open reading frame named the "U exon," located on the adenovirus (Ad) l-strand (for leftward transcription) between the early E3 region and the fiber gene, is conserved in mastadenoviruses. We have observed that Ad5 mutants with large deletions in E3 that infringe on the U exon display a mild growth defect, as well as an aberrant Ad E2 DNA-binding protein (DBP) intranuclear localization pattern and an apparent failure to organize replication centers during late infection. Mutants in which the U exon DNA is reconstructed have a reversed phenotype. Chow et al. (L. T. Chow et al., J. Mol. Biol. 134:265-303, 1979) described mRNAs initiating in the region of the U exon and spliced to downstream sequences in the late DBP mRNA leader and the DBP-coding region. We have cloned this mRNA (as cDNA) from Ad5 late mRNA; the predicted protein is 217 amino acids, initiating in the U exon and continuing in frame in the DBP leader and in the DBP-coding region but in a different reading frame from DBP. Polyclonal and monoclonal antibodies generated against the predicted U exon protein (UXP) showed that UXP is approximately 24K in size by immunoblot and is a late protein. At 18 to 24 h postinfection, UXP is strongly associated with nucleoli and is found throughout the nucleus; later, UXP is associated with the periphery of replication centers, suggesting a function relevant to Ad DNA replication or RNA transcription. UXP is expressed by all four species C Ads. When expressed in transient transfections, UXP complements the aberrant DBP localization pattern of UXP-negative Ad5 mutants. Our data indicate that UXP is a previously unrecognized protein derived from a novel late l-strand transcription unit.

Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Publishing Authors By Initials

AE Tollefson,B Ying,K Doronin,PD Sidor,WS Wold,AE Tollefson,B Ying,K Doronin,PD Sidor,WS Wold,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 12918-26

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 19

MONTH: 09

YEAR: 2007

Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: physiology

Chemical & Substance for Abstract: Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit.

AFFILIATION: Department of Molecular Microbiology and Immunology, St. Louis University Health Sciences Center, 1100 South Grand Blvd., St. Louis, MO 63104, USA.

Country: United States

AGENCY: United States NCI

GRANT: R01 CA118022

ACRONYM: CA

MEDLINETA: J Virol

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