Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library.

Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library. Research Abstract Details 

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Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library. Abstract Text:

Dan Jin,Wei Lu,Shuzhen Ping,Wei Zhang,Jian Chen,Baoqing Dun,Ruiqiang Ma,Zhonglin Zhao,Jiying Sha,Liang Li,Zhirong Yang,Ming Chen,Min Lin,Dan Jin,Wei Lu,Shuzhen Ping,Wei Zhang,Jian Chen,Baoqing Dun,Ruiqiang Ma,Zhonglin Zhao,Jiying Sha,Liang Li,Zhirong Yang,Ming Chen,Min Lin,

Glyphosate, a powerful nonselective herbicide, acts as an inhibitor of the activity of the enzyme 5-enoylpyruvylshikimate-3-phosphate synthase (EPSPS) encoded by the aroA gene involved in aromatic amino acid biosynthesis. An Escherichia coli mutant AKM4188 was constructed by insertion a kanamycin cassette within the aroA coding sequence. The mutant strain is an aromatic amino acids auxotroph and fails to grow on M9 minimal media due to the inactive aroA. A DNA metagenomic library was constructed with samples from a glyphosate-polluted area and was screened by using the mutant AKM4188 as recipient. Three plasmid clones, which restored growth to the aroA mutant in M9 minimal media supplemented with chloramphenicol, kanamycin, and 50 mM: glyphosate, were obtained from the DNA metagenomic library. One of them, which conferred glyphosate tolerance up to 150 mM: , was further characterized. The cloned fragment encoded a polypeptide, designated RD, sharing high similarity with other Class II EPSPS proteins. A His-tagged RD fusion protein was produced into E. coli to characterize the enzymatic properties of the RD EPSP protein.

Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library. Publishing Authors By Initials

D Jin,W Lu,S Ping,W Zhang,J Chen,B Dun,R Ma,Z Zhao,J Sha,L Li,Z Yang,M Chen,M Lin,D Jin,W Lu,S Ping,W Zhang,J Chen,B Dun,R Ma,Z Zhao,J Sha,L Li,Z Yang,M Chen,M Lin,

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Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current microbiology

VOLUME: 55

Page Numbers: 350-5

Journal Abbreviation: Curr. Microbiol.

ISSN: 0343-8651

DAY: 26

MONTH: 07

YEAR: 2007

Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library.

AFFILIATION: College of Life Sciences, Key Laboratory of Bio-Resources and Eco-Environment, The Ministry of Education, Sichuan University, 610064, Chengdu, China.

Country: United States

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MEDLINETA: Curr Microbiol

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