Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli.

Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli. Research Abstract Details 

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Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli. Abstract Text:

Xiaoyu Wang,Ming Li,Jiahuan Zhang,Yan Zhang,Guiying Zhang,Jinsheng Wang,Xiaoyu Wang,Ming Li,Jiahuan Zhang,Yan Zhang,Guiying Zhang,Jinsheng Wang,

In the current study, we identified a key functional region in harpins from Xanthomonas that suppressed protein aggregation and mediated its expression in E. coli. Our data suggested that the presence of two common features in harpins [Wei et al. (1992) Science 257:85-88], namely, high glycine content and lack of cysteine residues, were not sufficient for Xanthomonas to elicit hypersensitive response (HR) activity or heat stability. Additionally, bioinformatic analyses revealed that the secondary structure of a conserved N-terminal region consisting of 12 highly hydrophilic amino acids (QGISEKQLDQLL) was alpha-helical. Following site-directed mutagenesis deletion of this region, the three mutated harpin proteins, in cultures induced at 37 degrees C, failed to elicit a HR in tobacco leaves. However, at 24 degrees C, two mutated harpins retained the ability to elicit HR, albeit with lower expression levels than that noted with the wild-type. SDS-PAGE and Western blot data suggested the HpaG mutant protein was found almost entirely in the inclusion body. These data demonstrated that these conserved amino acid residues played a critical role in protein aggregation and inclusion body formation in harpins from Xanthomonas.

Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli. Publishing Authors By Initials

X Wang,M Li,J Zhang,Y Zhang,G Zhang,J Wang,X Wang,M Li,J Zhang,Y Zhang,G Zhang,J Wang,

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Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology reports

VOLUME: 34

Page Numbers: 189-98

Journal Abbreviation: Mol. Biol. Rep.

ISSN: 0301-4851

DAY: 19

MONTH: 12

YEAR: 2006

Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli.

AFFILIATION: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.

Country: Netherlands

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MEDLINETA: Mol Biol Rep

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