Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line.

Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Research Abstract Details 

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Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Abstract Text:

Matthias Christgen,Matthias Ballmaier,Henriette Bruchhardt,Reinhard von Wasielewski,Hans Kreipe,Ulrich Lehmann,Matthias Christgen,Matthias Ballmaier,Henriette Bruchhardt,Reinhard von Wasielewski,Hans Kreipe,Ulrich Lehmann,

"Side population" (SP) cells, which pump out the fluorescent dye H33342 via the ABCG2 transporter, define a putative stem/progenitor cell population in the mammary gland. Breast cancer SP cells recently isolated from the MCF-7 cell line possess similar properties and may represent stem cell-like cancer cells. This study extends SP cell analysis to a broad panel of human breast cancer cell lines and investigates the expression of differentiation-associated markers in isolated cancer SP cells. Expression of ABCG2 was determined in 16 breast cancer cell lines by quantitative RT-PCR, Western blotting and immunohistochemistry. Subsequently, all cell lines were screened for the presence of SP cells. Human breast cancer cell lines commonly express ABCG2. ABCG2-immunoreactivity was clearly restricted to rare cancer cells in several cell lines including Cal-51. Analysis of H33342-labeled Cal-51 cells revealed a small fraction of putative SP cells accounting for one percent of all cells. The genuine nature of Cal-51 SP cells was unambiguously verified by demonstrating a 30-fold increased ABCG2-expression in isolated Cal-51 SP cells. During in vitro expansion, Cal-51 SP cells generated heterologous non-SP (NSP) cells and ABCG2-expression declined dramatically. In contrast, NSP cells failed to sustain proliferation. Freshly isolated Cal-51 SP cells also exhibited increased expression of Muc1 and CALLA. Noteworthy, non-malignant mammary epithelial SP cells lack these differentiation markers, highlighting fundamental differences between non-malignant and breast cancer-derived SP cells. In summary, we established Cal-51 SP cells as a novel in vitro model to study differential gene expression in breast cancer-derived SP and NSP cells.

Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Publishing Authors By Initials

M Christgen,M Ballmaier,H Bruchhardt,R von Wasielewski,H Kreipe,U Lehmann,M Christgen,M Ballmaier,H Bruchhardt,R von Wasielewski,H Kreipe,U Lehmann,

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Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular and cellular biochemistry

VOLUME: 306

Page Numbers: 201-12

Journal Abbreviation: Mol. Cell. Biochem.

ISSN: 0300-8177

DAY: 28

MONTH: 07

YEAR: 2007

Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line.

AFFILIATION: Institute of Pathology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany. Christgen.Matthias@MH-Hannover.de

Country: Netherlands

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MEDLINETA: Mol Cell Biochem

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