Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia.

Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia. Research Abstract Details 

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Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia. Abstract Text:

Tomasz Trombik,Michal Jasinski, Crouzet,Marc Boutry,

ATP-binding cassette transporters of the pleiotropic drug resistance (PDR) subfamily are composed of five clusters. We have cloned a gene, NpPDR2, belonging to the still uncharacterized cluster IV from Nicotiana plumbaginifolia. NpPDR2 transcripts were found in the roots and mature flowers. In the latter, NpPDR2 expression was restricted to the style and only after pollination. A 1.5-kb genomic sequence containing the putative NpPDR2 transcription promoter was fused to the beta-glucuronidase reporter gene. The GUS expression pattern confirmed the RT-PCR results that NpPDR2 was expressed in roots and the flower style and showed that it was localized around the conductive tissues. Unlike other PDR genes, NpPDR2 expression was not induced in leaf tissues by none of the hormones typically involved in biotic and abiotic stress response. Moreover, unlike NpPDR1 known to be involved in biotic stress response, NpPDR2 expression was not induced in the style upon Botrytis cinerea infection. In N. plumbaginifolia plants in which NpPDR2 expression was prevented by RNA interference, no unusual phenotype was observed, including at the flowering stage, which suggests that NpPDR2 is not essential in the reproductive process under the tested conditions.

Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia. Publishing Authors By Initials

T Trombik,M Jasinski,J Crouzet,M Boutry,

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Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant molecular biology

VOLUME: 66

Page Numbers: 165-75

Journal Abbreviation: Plant Mol. Biol.

ISSN: 0167-4412

DAY: 22

MONTH: 11

YEAR: 2007

Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia. Information

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LANGUAGE: eng

NlmUniqueID: 9106343

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Grant and Affiliation Information for Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia.

AFFILIATION: Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud, 5-15, 1348, Louvain-la-Neuve, Belgium, marc.boutry@uclouvain.be.

Country: Netherlands

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MEDLINETA: Plant Mol Biol

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