Identification of a chloride ion binding site in Na+/Cl -dependent transporters.

Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Abstract Text:

Lucy R Forrest,Sotiria Tavoulari,Yuan-Wei Zhang,Gary Rudnick,Barry Honig,

The recent determination of the crystal structure of the leucine transporter from Aquifex aeolicus (aaLeuT) has provided significant insights into the function of neurotransmitter:sodium symporters. Transport by aaLeuT is Cl(-) independent, whereas many neurotransmitter:sodium symporters from higher organisms depend on Cl(-) ions. However, the only Cl(-) ion identified in the aaLeuT structure interacts with nonconserved residues in extracellular loops, and thus the relevance of this binding site is unclear. Here, we use calculations of pK(A)s and homology modeling to predict the location of a functionally important Cl(-) binding site in serotonin transporter and other Cl(-)-dependent transporters. We validate our model through the site-directed mutagenesis of residues predicted to coordinate the Cl(-) ion and through the observation of sequence conservation patterns in other Cl(-)-dependent transporters. The proposed site is located midway across the membrane and is formed by residues from transmembrane helices 2, 6, and 7. It is close to the Na1 sodium binding site, thus providing an explanation for the coupling of Cl(-) and Na(+) ions during transport. Other implications of the model are also discussed.

Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Publishing Authors By Initials

LR Forrest,S Tavoulari,YW Zhang,G Rudnick,B Honig,

For similar inorganic chemicals: electrolytes: ions: cations: cations, monovalent: protons research abstracts see: inorganic chemicals: electrolytes: ions: cations: cations, monovalent: protons research

PUBMED ID PMID:

MEDLINE DATE:

Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 12761-6

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 24

MONTH: 07

YEAR: 2007

Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Keywords Mesh Terms:

KEYWORDS: Protons

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification of a chloride ion binding site in Na+/Cl -dependent transporters. Information

Substance Name: cif nucleoside transporter

Registry Number: 0

Grant and Affiliation Information for Identification of a chloride ion binding site in Na+/Cl -dependent transporters.

AFFILIATION: Howard Hughes Medical Institute, Center for Computational Biology and Bioinformatics, and Department of Biochemistry and Molecular Biophysics, Columbia University, 1130 St. Nicholas Avenue, Room 815, New York, NY 10032, USA.

Country: United States

AGENCY: United States NIDA

GRANT: DA 007295

ACRONYM: DA

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of a chloride ion binding site in Na+/Cl -dependent transporters Related Publications

• A BIOCHEMICAL MECHANISM BY WHICH ADRENERGIC MEDIATORS MODIFY CARDIAC CONTRACTION.
• Acne arising in an epidermal nevus.
• Beta-thromboglobulin and platelet factor 4 levels in post-myocardial infarction patients with major depression.
• Cell-to-cell contact and extracellular matrix.
• Comparative study of generalized born models: Born radii and peptide folding.
• Diaper rash in a young infant.
• Electrocardiograms of more than routine interest. The electrocardiogram in a patient with mitral atresia and single functional ventricle.
• Etretinate therapy for generalized pustular psoriasis in children.
• Experimental fusion of the naturally cleft, embryonic chick palate.
• Fluvoxamine versus fluoxetine in major depressive episode: a double-blind randomised comparison.
• Identification of a chloride ion binding site in Na+/Cl -dependent transporters.
• Intention tremor of the head in patients with essential tremor.
• Labelling alcoholic drinks: percentage proof, original gravity, percentage alcohol or standard drinks?
• Laboratory biomarkers in Alzheimer's disease.
• Local quality assessment in homology models using statistical potentials and support vector machines.
• Loop modeling: Sampling, filtering, and scoring.
• Nuclear localization and cytosolic overexpression of LASP-1 correlates with tumor size and nodal-positivity of human breast carcinoma.
• Nuclear spin resonance of (129)Xe doped with O(2).
• Prediction of side-chain conformations on protein surfaces.
• Protein structure space is much more than the sum of its folds.
• Salt effects on polyelectrolyte-ligand binding: comparison of Poisson-Boltzmann, and limiting law/counterion binding models.
• Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease.
• Secondary ion quadrupole mass spectrometer for depth profiling-design and performance evaluation.
• Sequence and structural determinants of strand swapping in cadherin domains: do all cadherins bind through the same adhesive interface?
• Structural refinement of protein segments containing secondary structure elements: Local sampling, knowledge-based potentials, and clustering.
• Treatment of post-myocardial infarction depressive disorder.
• [Chest pain due to a panic disorder: the significance of a multidisciplinary approach]
• [In Process Citation]
• [Mortality in pregnant and newly delivered women due to psychiatric disorders]
• [The placing of an unborn child under formal supervision where there is doubt about the safety of the home situation]