Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection.

Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection. Research Abstract Details 

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Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection. Abstract Text:

Mingjin Liang,Zhaoguang Zheng,Ying Yuan,Lingyi Kong,Yunheng Shen,Runhui Liu,Chuan Zhang,Weidong Zhang,Mingjin Liang,Zhaoguang Zheng,Ying Yuan,Lingyi Kong,Yunheng Shen,Runhui Liu,Chuan Zhang,Weidong Zhang,

High-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-ESI/MS) and evaporative light scattering detection (HPLC-ELSD), respectively, has been performed for the simultaneous identification and quantification of six C(21) steroid saponins, including cynanversicoside A, B, D, G, glaucoside C and glaucogenin C-3-O-beta-d-thevetopyranoside in Radix Cynanchi Atrati. The extraction of the C(21) steroidal saponins was performed using a B-811 Buchi Universal Extraction System in Warm Solvent Mode, and the analyte was concentrated by column chromatography before HPLC analysis. The chromatographic separation was performed on an Agilent Zorbax Extend C(18) analytical column efficiently using gradient elution with acetonitrile and water. The method was validated with acceptable linearities (r > 0.9991) and recoveries (98.2-101.3%). The limits of detection of the C(21) steroid saponins were from 0.2 microg for glaucogenin C-3-O-beta-d-thevetopyranoside to 0.5 microg for cynanversicoside B. The intra- and inter-day precisions of the method were evaluated and were less than 5.0%. The method was successfully used to analyse 20 batches of Radix Cynanchi Atrati. The content of C(21) steroid saponins in the plant material varied significantly from habitat to habitat, confirming the necessity to control the quality of Radix Cynanchi Atrati during its preparation and application in the clinic.

Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection. Publishing Authors By Initials

M Liang,Z Zheng,Y Yuan,L Kong,Y Shen,R Liu,C Zhang,W Zhang,M Liang,Z Zheng,Y Yuan,L Kong,Y Shen,R Liu,C Zhang,W Zhang,

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Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Phytochemical analysis : PCA

VOLUME: 18

Page Numbers: 428-35

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ISSN: 0958-0344

DAY: 30

MONTH: Sep

YEAR: 2007

Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection. Information

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LANGUAGE: eng

NlmUniqueID: 9200492

Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection. Keywords Mesh Terms:

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Grant and Affiliation Information for Identification and quantification of C21 steroidal saponins from Radix Cynanchi Atrati by high-performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection.

AFFILIATION: School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, People's Republic of China.

Country: England

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MEDLINETA: Phytochem Anal

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