Identification and possible roles of three types of endopeptidase from germinated wheat seeds.

Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Abstract Text:

K Sutoh,H Kato,T Minamikawa,

Little or no endopeptidase activity was detected in extracts of dry mature wheat seeds, but when they were allowed to imbibe water in darkness, the activity expressed per seedling increased notably after d 1, reached a maximum on d 3 and then decreased. Two major endopeptidases, named WEP-1 and WEP-2, were present in the 50-70% saturated ammonium sulfate fraction of d-3 seedlings, and could be separated by hydrophobic column chromatography. WEP-1 was further purified and identified as a 31-kDa polypeptide that was immunoreactive to antiserum raised against REP-1, a major rice cysteine endopeptidase. Experiments with proteinase inhibitors revealed that WEP-1 and WEP-2 are cysteine and serine endopeptidases, respectively. The two enzymes differed in substrate specificity, pH dependence, and the ability to digest major wheat seed proteins. Determination of its amino-terminal amino acid sequence indicated the similarity of WEP-1 to other cereal cysteine endopeptidases which are involved in the digestion of seed storage proteins. The expression of WEP-1 in de-embryonated seeds was induced in the presence of gibberellic acid and its effect was eliminated by abscisic acid. In addition to WEP-1 and WEP-2, a legumain-like asparaginyl endopeptidase was identified in the extract of seedlings on hydrophobic chromatography. The asparaginyl endopeptidase may function in the early step of mobilization of wheat storage proteins in germinated seeds.

Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Publishing Authors By Initials

K Sutoh,H Kato,T Minamikawa,

For similar plants: plant families and groups: angiosperms: poaceae: triticum research abstracts see: plants: plant families and groups: angiosperms: poaceae: triticum research

PUBMED ID PMID:

MEDLINE DATE:

Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 126

Page Numbers: 700-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1999

Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Keywords Mesh Terms:

KEYWORDS: Triticum

MESH TERMS: genetics

Chemical & Substance for Abstract: Identification and possible roles of three types of endopeptidase from germinated wheat seeds. Information

Substance Name: serine endopeptidase

Registry Number: EC 3.4.99.-

Grant and Affiliation Information for Identification and possible roles of three types of endopeptidase from germinated wheat seeds.

AFFILIATION: Department of Biological Sciences, Tokyo Metropolitan University, Minami-ohsawa, Hachioji, Tokyo, 192-0397, Japan.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification and possible roles of three types of endopeptidase from germinated wheat seeds Related Publications

• 3-Dehydroquinate synthase in germinating Phaseolus mungo seedlings.
• Aminocarbonylation route to tolvaptan.
• Identification and possible roles of three types of endopeptidase from germinated wheat seeds.