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Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12.

Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Research Abstract Details 

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  • Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Abstract Text:

    h hommaH Homma,t kobayashiT Kobayashi,y itoY Ito,i kudoI Kudo,k inoueK Inoue,h ikedaH Ikeda,m sekiguchiM Sekiguchi,s nojimaS Nojima,

    E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for the lysophospholipase L2 activity was designated as pld B. On the same hybrid plasmid another gene (pld A) coding for detergent-resistant phospholipase A (DR-phospholipase A) was also identified. These facts together with the results of a Pl transduction experiment revealed that the pld B gene must be between the pld A and met E genes on the E. coli chromosome.

    Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Publishing Authors By Initials

    h hommaH Homma,t kobayashiT Kobayashi,y itoY Ito,i kudoI Kudo,k inoueK Inoue,h ikedaH Ikeda,m sekiguchiM Sekiguchi,s nojimaS Nojima,

    For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

    PUBMED ID PMID:

    MEDLINE DATE:

    Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of biochemistry

    VOLUME: 94

    Page Numbers: 2079-81

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Dec

    YEAR: 1983

    Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Keywords Mesh Terms:

    KEYWORDS: Plasmids

    MESH TERMS: genetics

    Chemical & Substance for Abstract: Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12. Information

    Substance Name: Lysophospholipase

    Registry Number: EC 3.1.1.5

    Grant and Affiliation Information for Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12.

    AFFILIATION:

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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