Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies.

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Abstract Text:

Anne-Laure Goenaga,Yu Zhou,Christine Legay,Houcine Bougherara,Lan Huang,Bin Liu,Daryl C Drummond,Dmitri B Kirpotin,Christian Auclair,James D Marks,Marie-Alix Poul,

To generate a panel of antibodies binding human breast cancers, a human single chain Fv phage display library was selected for rapid internalization into the SK-BR-3 breast cancer cell line. Thirteen unique antibodies were identified within the 55 cell binding antibodies studied, all of them showing specific staining of tumor cells compare to normal epithelial cells. Two of the antibodies bound the ErbB2 oncogene while 6 bound the tumor marker transferrin receptor (TfR). By developing a scFv immunoprecipitation method, we were able to use LC-MS/MS to identify the antigen bound by one of the antibodies (3GA5) as FPRP (prostaglandin F2alpha receptor-regulatory protein)/EWI-F/CD9P-1 (CD9 partner 1) an Ig superfamily member that has been described to interact directly with CD9 and CD81 tetraspanins and to be overexpressed in adherent cancer cell lines. Although the 3GA5 scFv had no direct anti-proliferative effect, intracellular expression of the scFv was able to knockdown CD9P-1 expression and could be used to further define the role of the tetraspanin system in proliferation and metastasis. Moreover, the 3GA5 scFv was rapidly internalized into breast tumor cells and could have potential for the targeted delivery of cytotoxic agents to breast cancers. This study is the proof of principle that the direct selection of phage antibody libraries on tumor cells can effectively lead to the identification and functional characterization of relevant tumor markers.

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Publishing Authors By Initials

AL Goenaga,Y Zhou,C Legay,H Bougherara,L Huang,B Liu,DC Drummond,DB Kirpotin,C Auclair,JD Marks,MA Poul,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

PUBMED ID PMID:

MEDLINE DATE:

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular immunology

VOLUME: 44

Page Numbers: 3777-88

Journal Abbreviation: Mol. Immunol.

ISSN: 0161-5890

DAY: 10

MONTH: 05

YEAR: 2007

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905289

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies. Information

Substance Name: Peptide Library

Registry Number: 0

Grant and Affiliation Information for Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies.

AFFILIATION: ENS Cachan Laboratoire de Biotechnologie et Pharmacologie Génétique Appliquée (LBPA), UMR CNRS 8113, 61 avenue du Président Wilson, 94235 Cachan Cedex, France.

Country: England

AGENCY: United States NCI

GRANT: P50-CA58207

ACRONYM: CA

MEDLINETA: Mol Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies Related Publications

• Diagnosis of Legg-Calvé-Perthes disease.
• Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies.