Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis.

Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. Research Abstract Details 

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Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. Abstract Text:

Laura L Grochowski,Huimin Xu,Robert H White,

Coenzyme F 420 is a hydride carrier cofactor functioning in methanogenesis. One step in the biosynthesis of coenzyme F 420 involves the coupling of 2-phospho- l-lactate (LP) to 7,8-didemethyl-8-hydroxy-5-deazaflavin, the F 420 chromophore. This condensation requires an initial activation of 2-phospho- l-lactate through a pyrophosphate linkage to GMP. Bioinformatic analysis identified an uncharacterized archaeal protein in the Methanocaldococcus jannaschii genome, MJ0887, which could be involved in this transformation. The predicted MJ0887-derived protein has domain similarity with other known nucleotidyl transferases. The MJ0887 gene was cloned and overexpressed, and the purified protein was found to catalyze the formation of lactyl-2-diphospho-5'-guanosine from LP and GTP. Kinetic constants were determined for the MJ0887-derived protein with both LP and GTP substrates and are as follows: V max = 3 micromol min (-1) mg (-1), GTP K M (app) = 56 microM, and k cat/ K M (app) = 2 x 10 (4) M (-1) s (-1) and LP K M (app) = 36 microM, and k cat/ K M (app) = 4 x 10 (4) M (-1) s (-1). The MJ0887 gene product has been designated CofC to indicate its involvement in the third step of coenzyme F 420 biosynthesis.

Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. Publishing Authors By Initials

LL Grochowski,H Xu,RH White,

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Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemistry

VOLUME: 47

Page Numbers: 3033-7

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 9

MONTH: 02

YEAR: 2008

Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis.

AFFILIATION: rhwhite@vt.edu.

Country: United States

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MEDLINETA: Biochemistry

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