Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period.

Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Research Abstract Details 

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Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Abstract Text:

S C Ho,S Wakatsuki,M Arioka,M Yamasaki,K Kitamoto,

To identify and analyze the function of proteins expressed in the growth cones, we have screened monoclonal antibodies raised against the preparation of the growth cone particles derived from fetal porcine brains and found a novel neuronal antigen, termed NP-190. Biochemical characterization of NP-190 demonstrated that it was an integral membrane protein with an apparent molecular weight of 190 kDa and that it was mainly expressed in fetal brains. Homologous antigens with molecular weights of 200 and 170 kDa were also identified in the fetal brain extracts of chickens and rats, respectively. Immunoblot experiments of brain extracts from chickens and rats in various stages of development indicated that the expression of NP-190 homologs was developmentally regulated; it began to appear and increased in the embryonic stage, then decreased to very low level in the adult brains. Immunostaining of cultured primary of neurons from the embryonic day 18 rat cerebral cortex demonstrated that rat NP-190 homolog localized in the cell bodies, axons and growth cones, but not in dendrites. Partial amino acid sequence analysis of affinity-purified NP-190 from fetal porcine brains demonstrated that it was a novel protein. These results suggest that NP-190 plays a distinct role in brain development.

Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Publishing Authors By Initials

SC Ho,S Wakatsuki,M Arioka,M Yamasaki,K Kitamoto,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 123

Page Numbers: 332-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1998

Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period. Information

Substance Name: Nerve Tissue Proteins

Registry Number: 0

Grant and Affiliation Information for Identification and characterization of porcine NP-190, a novel protein that is specifically expressed in the axonal membrane during the embryonic period.

AFFILIATION: Department of Biotechnology, The University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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