Identification and characterization of canine dendritic cells generated in vivo.

Identification and characterization of canine dendritic cells generated in vivo. Research Abstract Details 

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Identification and characterization of canine dendritic cells generated in vivo. Abstract Text:

Marco Mielcarek,Kristin A Kucera,Richard Nash,Beverly Torok-Storb,Hilary J McKenna,Marco Mielcarek,Kristin A Kucera,Richard Nash,Beverly Torok-Storb,Hilary J McKenna,

Emerging evidence suggests that host dendritic cells (DC) initiate and regulate graft-versus-host and graft-versus-tumor reactions after allogeneic hematopoietic cell transplantation (HCT). Even though decades of experimentation in the preclinical canine HCT model have substantially improved our understanding of the biology and safety of HCT in human patients, the in vivo phenotype of potent antigen-presenting cells in dogs is poorly defined. Therefore, peripheral blood leukocytes were obtained from dogs treated with recombinant human Flt3-ligand and phenotypically distinct cell populations, including putative DC, were purified by 4-color flow-cytometry and tested for their stimulatory potential in allogeneic mixed lymphocyte cultures (MLC). Cells characterized by surface expression of CD11c and HLA-DR, and absence of expression of CD14 and DM5, a marker of mature granulocytes, were found to be highly potent stimulators in allogeneic MLC. In contrast, all other immunophenotypically different cell populations tested had either weak or absent allostimulatory potential. Transmission electron microscopy of CD11c+/HLA-DR+/CD14-/DM5- cells revealed the morphology similar to that described for DC in humans and ex vivo-generated canine DC, including long cytoplasmic extensions, discrete lysosomes, and an abundant Golgi apparatus and endoplasmatic reticulum. In summary, CD11c+/HLA-DR+/CD14-/DM5- cells obtained from canine peripheral blood have functional and morphologic characteristics similar to those of human myeloid DC.

Identification and characterization of canine dendritic cells generated in vivo. Publishing Authors By Initials

M Mielcarek,KA Kucera,R Nash,B Torok-Storb,HJ McKenna,M Mielcarek,KA Kucera,R Nash,B Torok-Storb,HJ McKenna,

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Identification and characterization of canine dendritic cells generated in vivo. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biology of blood and marrow transplantation : jour

VOLUME: 13

Page Numbers: 1286-93

Journal Abbreviation: Biol. Blood Marrow Transplant.

ISSN: 1083-8791

DAY: 7

MONTH: 09

YEAR: 2007

Identification and characterization of canine dendritic cells generated in vivo. Information

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LANGUAGE: eng

NlmUniqueID: 9600628

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Grant and Affiliation Information for Identification and characterization of canine dendritic cells generated in vivo.

AFFILIATION: Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA. mmielcar@fhcrc.org

Country: United States

AGENCY: United States NIDDK

GRANT: DK064715

ACRONYM: DK

MEDLINETA: Biol Blood Marrow Transplant

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