Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation.

Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Research Abstract Details 

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Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Abstract Text:

Hai-Chang Wang,Hai-Feng Zhang,Wen-Yi Guo,Hui Su,Kun-Ru Zhang,Qiu-Xia Li,Wenli Yan,Xin L Ma,Bernard L Lopez,Theodore A Christopher,Feng Gao,

OBJECTIVES: Our previous study has shown that slow or "controlled" reperfusion for the ischemic heart reduces cardiomyocyte injury and myocardial infarction, while the mechanisms involved are largely unclear. In this study, we tested the hypothesis that enhancement of survival and prevention of apoptosis in hypoxic/reoxygenated cardiomyocytes by hypoxic postconditioning (HPC) are associated with the reduction in peroxynitrite (ONOO(-)) formation induced by hypoxia/reoxygenation (H/R). METHODS: Isolated adult rat cardiomyocytes were exposed to 2 h of hypoxia followed by 3 h of reoxygenation. After 2 h of hypoxia the cardiomyocytes were either abruptly reperfused with pre-oxygenized culture medium or postconditioned by two cycles of 5 min of brief reoxygenation and 5 min of re-hypoxia followed by 160 min of abrupt reoxygenation. RESULTS: H/R resulted in severe injury in cardiomyocytes as evidenced by decreased cell viability, increased LDH leakage in the culture medium, increased apoptotic index (P values all less than 0.01 vs. normoxia control group) and DNA ladder formation, which could be significantly attenuated by HPC treatment applied before the abrupt reoxygenation (P < 0.05 vs. H/R group). In addition, H/R induced a significant increase in ONOO(-) formation as determined by nitrotyrosine content in cardiomyocytes (P < 0.01 vs. normoxia control). Treatment with the potent ONOO(-) scavenger uric acid (UA) at reoxygenation significantly decreased ONOO(-) production and protected myocytes against H/R injury, whereas the same treatment with UA could not further enhance myocyte survival in HPC group (P > 0.05 vs. HPC alone). Statistical analysis showed that cell viability closely correlated inversely with myocyte ONOO(-) formation (P < 0.01). CONCLUSION: These data demonstrate that hypoxic postconditioning protects myocytes against apoptosis following reoxygenation and enhances myocytes survival, which is partly attributable to the reduced ONOO(-) formation following reoxygenation.

Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Publishing Authors By Initials

HC Wang,HF Zhang,WY Guo,H Su,KR Zhang,QX Li,W Yan,XL Ma,BL Lopez,TA Christopher,F Gao,

For similar heterocyclic compounds: alkaloids: xanthines: uric acid research abstracts see: heterocyclic compounds: alkaloids: xanthines: uric acid research

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Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Apoptosis : an international journal on programmed

VOLUME: 11

Page Numbers: 1453-60

Journal Abbreviation: Apoptosis

ISSN: 1360-8185

DAY: 3

MONTH: Aug

YEAR: 2006

Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9712129

Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Keywords Mesh Terms:

KEYWORDS: Uric Acid

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation. Information

Substance Name: L-Lactate Dehydrogenase

Registry Number: EC 1.1.1.27

Grant and Affiliation Information for Hypoxic postconditioning enhances the survival and inhibits apoptosis of cardiomyocytes following reoxygenation: role of peroxynitrite formation.

AFFILIATION: Department of Cardiology and Department of Physiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 63828

ACRONYM: HL

MEDLINETA: Apoptosis

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