Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer.

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Abstract Text:

Q Wang,M Williamson,S Bott,N Brookman-Amissah,A Freeman,J Nariculam,M J F Hubank,A Ahmed,J R Masters,

Oligoarray analysis of a matched pair of prostate cancer and normal cell lines derived from the same radical prostatectomy specimen identified 113 candidate hypomethylated genes that were overexpressed in the cancer cells and contained CpG islands. Hypomethylation of wingless-related MMTV integration site 5A (WNT5A), S100 calcium-binding protein P (S100P) and cysteine-rich protein 1(CRIP1) was confirmed in the cancer cells by bisulfite sequencing. Treatment of the corresponding normal prostate epithelial cells 1542-NPTX with the DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-aza-CdR) induced higher levels of mRNA expression and partial loss of methylation on these genes. Primary prostate cancers were tested using methylation-specific polymerase chain reaction. WNT5A was hypomethylated in 11/17 (65%) tumors, S100P in 8/16 (50%) and CRIP1 in 13/20 (65%). Bisulfite sequencing of a section of the 5' untranslated region (UTR) of WNT5A revealed that three CpG sites (15, 24 and 35) were consistently methylated (93%) in the normal cell line and normal tissues, but not in the prostate cancer cell line and eight primary prostate cancers. Multiple putative binding sites for the transcription factors SP1 and AP-2 were found adjacent to CpG sites 15 and 24. A putative c-Myb binding site was located within the CpG site 35. Anti-c-Myb antibody co-precipitation with WNT5A was methylation-sensitive in 1542-NPTX cells. It is likely that an epigenetic mechanism regulates WNT5A expression in prostate cancer.

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Publishing Authors By Initials

Q Wang,M Williamson,S Bott,N Brookman-Amissah,A Freeman,J Nariculam,MJ Hubank,A Ahmed,JR Masters,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Oncogene

VOLUME: 26

Page Numbers: 6560-5

Journal Abbreviation: Oncogene

ISSN: 0950-9232

DAY: 7

MONTH: 05

YEAR: 2007

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8711562

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer.

AFFILIATION: Prostate Cancer Research Centre, Institute of Urology and Nephrology, University College London, and Department of Histopathology, University College London Hospitals Trust, UK.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Oncogene

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer Related Publications

• A hypervariable region within the 3' cis-acting element of the murine coronavirus genome is nonessential for RNA synthesis but affects pathogenesis.
• A modest proposal: bring back indentured servitude.
• A study of the adult mosquito population of a northern Ohio woods.
• Anti-suicidal and Self-harm Properties of Lithium Carbonate.
• Continuous-wave 4.3-mum intracavity difference frequency generation in an optical parametric oscillator.
• Contrasting experiences and perceptions of women and men physician graduates of Stanford University School of Medicine.
• Decline in verbal memory in non-demented older adults.
• Diagnosis of hemorrhagic disorders.
• Diminished FAD binding in the Y459H and V492E Antley-Bixler syndrome mutants of human cytochrome P450 reductase.
• Duodenal-colic fistula as a complication of regional ileitis.
• Eotopic pregnancy; a report of 107 cases.
• Evaluation of head trauma: efficacy of skull films.
• Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function.
• Gemcitabine and Carboplatin in advanced non small-cell lung cancer: a review.
• Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome.
• Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancer.
• Immobilized gradients of epidermal growth factor promote accelerated and directed keratinocyte migration.
• Isolation of avian paramyxovirus 1 from a patient with a lethal case of pneumonia.
• Mechanisms of Abeta mediated neurodegeneration in Alzheimer's disease.
• Oxygen metabolism by endothelial nitric-oxide synthase.
• Oxygen metabolism by neuronal nitric-oxide synthase.
• Primary acquired agammaglobulinemia, with granulomas of the skin and internal organs.
• Pulse oximetry use during physical and mechanical restraints.
• Rapid prototype fabrication of a monolithic hearing instrument housing with an integrally-fabricated faceplate.
• Rationale of sex steroid replacement in the neutral gender.
• Senile cataracts and aging changes in human proteins.
• The haemocompatibility of polyurethane-hyaluronic acid copolymers.
• The role of a conserved serine residue within hydrogen bonding distance of FAD in redox properties and the modulation of catalysis by Ca2+/calmodulin of constitutive nitric-oxide synthases.
• Total gastrectomy for benign gastric ulcer.
• We just clicked.