Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I.

Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Research Abstract Details 

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Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Abstract Text:

Laura A Maile,Byron E Capps,Yan Ling,Gang Xi,David R Clemmons,

IGF-I stimulation of smooth muscle cell (SMC) migration and proliferation requires alphaVbeta3 ligand occupancy. We hypothesized that changes in the levels of extracellular matrix proteins induced by alterations in glucose concentrations may regulate the ability of SMCs to respond to IGF-I. IGF-I stimulated migration and proliferation of SMCs that had been maintained in 25 mM glucose containing media, but it had no stimulatory effect when tested using SMCs that had been grown in 5 mM glucose. IGF-I stimulated an increase in Shc phosphorylation and enhanced activation of the MAPK pathway in SMCs grown in 25 mM glucose, whereas in cells maintained in 5 mM glucose, IGF-I had no effect on Shc phosphorylation, and the MAPK response to IGF-I was markedly reduced. In cells grown in 25 mM glucose, the levels of alphaVbeta3 ligands, e.g. osteopontin, vitronectin, and thrombospondin, were all significantly increased, compared with cells grown in 5 mM glucose. The addition of these alphaVbeta3 ligands to SMCs grown in 5 mM glucose was sufficient to permit IGF-I-stimulated Shc phosphorylation and downstream signaling. Because we have shown previously that alphaVbeta3 ligand occupancy is required for IGF-I-stimulated Shc phosphorylation and stimulation of SMC growth, our data are consistent with a model in which 25 mM glucose stimulates increases in the concentrations of these extracellular matrix proteins, thus enhancing alphaVbeta3 ligand occupancy, which leads to increased Shc phosphorylation and enhanced cell migration and proliferation in response to IGF-I.

Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Publishing Authors By Initials

LA Maile,BE Capps,Y Ling,G Xi,DR Clemmons,

For similar proteins: blood proteins: vitronectin research abstracts see: proteins: blood proteins: vitronectin research

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Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Endocrinology

VOLUME: 148

Page Numbers: 2435-43

Journal Abbreviation:

ISSN: 0013-7227

DAY: 25

MONTH: 01

YEAR: 2007

Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375040

Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Keywords Mesh Terms:

KEYWORDS: Vitronectin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I. Information

Substance Name: Extracellular Signal-Regulated MAP Kinas

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I.

AFFILIATION: Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599-7170, USA. laura_maile@med.unc.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL56850

ACRONYM: HL

MEDLINETA: Endocrinology

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