HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages.

HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages. Research Abstract Details 

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HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages. Abstract Text:

Jing Wang,Yong Gu,Lihong Wang,Xingyi Hang,Yan Gao,Hangyan Wang,Chenggang Zhang,Jing Wang,Yong Gu,Lihong Wang,Xingyi Hang,Yan Gao,Hangyan Wang,Chenggang Zhang,

This study is a part of the HUPO Brain Proteome Project (BPP) pilot study, which aims at obtaining a reliable database of mouse brain proteome, at the comparison of techniques, laboratories, and approaches as well as at preparing subsequent proteome studies of neurologic diseases. The C57/Bl6 mouse brains of three developmental stages at embryonic day 16 (E16), postnatal day 7 (P7), and 8 wk (P56) (n = 5 in each group) were provided by the HUPO BPP executive committee. The whole brain proteins of each animal were individually prepared using 2-DE coupled with PDQuest software analysis. The protein spots representing developmentally related or stably expressed proteins were then prepared with in-gel digestion followed with MALDI-TOF/TOF MS/MS and analyzed using the MASCOT search engines to search the Swiss-Prot or NCBInr database. The 2-DE gel maps of the mouse brains of all of the developmental stages were obtained and submitted to the Data Collection Centre (DCC). The proteins alpha-enolase, stathmin, actin, C14orf166 homolog, 28,000 kDa heat- and acid-stable phosphoprotein, 3-mercaptopyruvate sulfurtransferase and 40 S ribosomal protein S3a were successfully identified. A further Western blotting analysis demonstrated that enolase is a protein up-regulated in the mouse brain from embryonic stage to adult stage. These data are helpful for understanding the proteome changes in the development of the mouse brain.

HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages. Publishing Authors By Initials

J Wang,Y Gu,L Wang,X Hang,Y Gao,H Wang,C Zhang,J Wang,Y Gu,L Wang,X Hang,Y Gao,H Wang,C Zhang,

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HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 7

Page Numbers: 4008-15

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 31

MONTH: Nov

YEAR: 2007

HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages. Information

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LANGUAGE: eng

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Grant and Affiliation Information for HUPO BPP pilot study: a proteomics analysis of the mouse brain of different developmental stages.

AFFILIATION: Department of Neurobiology, Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Beijing, China.

Country: Germany

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MEDLINETA: Proteomics

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