Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase.

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Abstract Text:

S Takahashi,K Takahashi,T Kaneko,H Ogasawara,S Shindo,M Kobayashi,

The existence of human renin-binding protein (RnBP) in the kidney has been shown by the isolation and characterization of a complex of porcine renin-human RnBP [S. Takahashi et al. (1985) J. Biochem. 97, 671-677]. However, the properties of the free form of human RnBP had not been understood, because of the limitation of materials. In the present study, we have expressed human RnBP in Escherichia coli JM 109 cells under the transcriptional control of taq promoter and purified it by conventional column chromatographies. The purified recombinant human RnBP (rhRnBP) exists as a dimer and inhibits porcine renin activity through formation of a complex of porcine renin with rhRnBP, the so-called high-molecular-weight renin. Moreover, the rhRnBP catalyzes the interconversion between N-acetyl-D-glucosamine (GlcNAc) and N-acetyl-D-Mannosamine (ManNAc) with the apparent Km values of 21.3 mM for GlcNAc and 12.8 mM for ManNAc, and 0.13 mM for effector ATP. ATP is essential for the GlcNAc 2-epimerase activity of human RnBP. These results indicate that the human RnBP is a GlcNAc 2-epimerase.

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Publishing Authors By Initials

S Takahashi,K Takahashi,T Kaneko,H Ogasawara,S Shindo,M Kobayashi,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

PUBMED ID PMID:

MEDLINE DATE:

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 125

Page Numbers: 348-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1999

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. Information

Substance Name: RENBP protein, human

Registry Number: EC 5.1.3.8

Grant and Affiliation Information for Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase.

AFFILIATION: Department of Bioengineering, Akita Research Institute of Food and Brewing, Sanuki, Arayamachi, Akita, 010-1623, Japan. saori@arif.pref.akita.jp

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase Related Publications

• A comparison of the emission efficiency of four common green fluorescence dyes after internalization into cancer cells.
• A novel mutation (Gln433Glu) in exon 12 combined with the G insertion in exon 13 causes severe factor XI deficiency in Japanese patients.
• A vicious cycle in the oral health status of schoolchildren in a primary school in rural Cambodia.
• Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes.
• Amygdala-dependent Mechanisms Underlying Memory Retrieval of Conditioned Taste Aversion.
• Bone regeneration through controlled release of bone morphogenetic protein-2 from 3-D tissue engineered nano-scaffold.
• Cadaveric Hands-on Workshop Using a Three-Dimensional High-Definition Television System.
• Comparison of the antivascular and cytotoxic activities of TZT-1027 (Soblidotin) with those of other anticancer agents.
• Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines.
• Heart Rate and Muscle Oxygenation during Hyperbaric Hyperoxia: 2343: Board #22 June 1 3:30 PM -5:00 PM.
• Hepatic uptake of bilirubin re-visited.
• MAMO, a maternal BTB/POZ-Zn-finger protein enriched in germline progenitors is required for the production of functional eggs in Drosophila.
• Maintenance behaviour and long-lasting insecticide-treated nets (LLITNs) previously introduced into Bourapar district, Khammouane province, Lao PDR.
• Management of the patients with early stage oral tongue cancers.
• Noise can delay and advance the collapse of spatiotemporal chaos.
• Phosphine oxides as efficient neutral coordinate-organocatalysts for stereoselective allylation of N-acylhydrazones.
• Porous imprinted polymer membranes prepared by phase separation in compressed liquid CO(2).
• Protective effect of alpha-tocopherol-6-O-phosphate against ultraviolet B-induced damage in cultured mouse skin.
• Pyothorax-associated lymphoma: A case showing transition from T-cell-rich polymorphic lesion to diffuse large B-cell lymphoma.
• Response of matrix metalloproteinase-9 to olfactory nerve injury.
• Single-pass BPM system of the Photon Factory storage ring.
• Spectral near-infrared fluorescence imaging of curved surfaces using projection reconstruction algorithms.
• Staphylococcus aureus cell-envelope antigens are localized in glomeruli from patients with IgA-related nephropathy.
• Steplike lattice deformation of single crystalline (La0.4Pr0.6)1.2Sr1.8Mn2O7 bilayered manganite.
• The Hes gene family: repressors and oscillators that orchestrate embryogenesis.
• The Odor Stick Identification Test for the Japanese (OSIT-J): Clinical Suitability for Patients Suffering from Olfactory Disturbance.
• Topical Application of gamma-Tocopherol Derivative Prevents UV-Induced Skin Pigmentation.
• Topical application of a novel, hydrophilic gamma-tocopherol derivative reduces photo-inflammation in mice skin.
• Which is the better pathological prognostic factor, the Nottingham histological grade or the Japanese nuclear grade? A large scale study with a long-term follow-up.
• Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance.