Human mammary cancer cell mutants with altered hormone receptor activity.

Human mammary cancer cell mutants with altered hormone receptor activity. Research Abstract Details 

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Human mammary cancer cell mutants with altered hormone receptor activity. Abstract Text:

H Ueda,Y Hagino,M Ono,M Kuwano,

We have recently isolated retinoic acid-resistant clones U-2 and U-3 from human breast cancer cell line MCF-7 (Ueda et al. (1985) Cancer Res. 45, 3332-3338). Growth of MCF-7 cells was found to be stimulated by estradiol but that of U-2 or U-3 was not. Cytosol from U-2 or U-3 cells contained no detectable estradiol receptor activity, whereas that from the parental MCF-7 cells showed estradiol receptor activity of 32 fmol/mg cytosol protein with a Kd of 2.6 X 10(-10) M by Scatchard analysis. Sucrose gradient centrifugation analysis of the cytosol fraction confirmed the presence of estradiol receptor activity in MCF-7 but not in U-2. Cytosol from MCF-7 and U-2 cells showed progesterone receptor activities of 106 fmol/mg protein with a Kd of 7.4 X 10(-10) M and 13 fmol/mg protein with a Kd of 9.9 X 10(-10) M, respectively. Addition of estradiol to the culture medium of the cells increased the level of progesterone receptor about 2-fold in MCF-7, but not in U-2. U-2 or U-3 cells showed about 5-fold higher resistance to an antiestrogen, tamoxifen, than MCF-7, and they were also 300- to 1,000-fold more resistant to other antiestrogens, epitiostanol and medroxyprogesterone, than MCF-7. The altered cellular sensitivity of U-2 or U-3 to the hormone antagonists is discussed in relation to the absence or presence of hormone receptors.

Human mammary cancer cell mutants with altered hormone receptor activity. Publishing Authors By Initials

H Ueda,Y Hagino,M Ono,M Kuwano,

For similar organic chemicals: hydrocarbons: hydrocarbons, cyclic: hydrocarbons, aromatic: benzene derivatives: benzylidene compounds: stilbenes: tamoxifen research abstracts see: organic chemicals: hydrocarbons: hydrocarbons, cyclic: hydrocarbons, aromatic: benzene derivatives: benzylidene compounds: stilbenes: tamoxifen research

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Human mammary cancer cell mutants with altered hormone receptor activity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 341-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1986

Human mammary cancer cell mutants with altered hormone receptor activity. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Human mammary cancer cell mutants with altered hormone receptor activity. Keywords Mesh Terms:

KEYWORDS: Tamoxifen

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Human mammary cancer cell mutants with altered hormone receptor activity. Information

Substance Name: Estradiol

Registry Number: 50-28-2

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Country: JAPAN

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MEDLINETA: J Biochem

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