Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms.

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Abstract Text:

Karen Fox,Elisangela Castanha,Alvin Fox,Charles Feigley,Deborah Salzberg,Karen Fox,Elisangela Castanha,Alvin Fox,Charles Feigley,Deborah Salzberg,Karen Fox,Elisangela Castanha,Alvin Fox,Charles Feigley,Deborah Salzberg,

Previously it was demonstrated that the levels of large particles (>2 micron) and associated bacterial cell envelope markers increase greatly on occupation in schools; it was hypothesized that the source of both was shed human skin. In the current work to test this hypothesis, room air cleaners were used to collect airborne dust (>50-100 mg) from occupied and unoccupied school rooms which was then subjected to proteomic analysis. Proteins were extracted from the dust and separated using two dimensional gel electrophoresis (2D GE). In situ digestion of protein spots with trypsin released peptides, which were subsequently analyzed by matrix assisted laser desorption/deionization, time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem mass spectrometry (MALDI-TOF-MS-MS). In Coomassie blue stained gels, a single spot generally dominated the 2D gels; this protein was identified by tandem mass spectrometry as K10 epithelial keratin. The results experimentally confirm previous anecdotal reports that human skin is readily shed into air and suggest that increased levels of microbial markers and large particles observed in occupied rooms are also derived from skin.

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Publishing Authors By Initials

K Fox,E Castanha,A Fox,C Feigley,D Salzberg,K Fox,E Castanha,A Fox,C Feigley,D Salzberg,K Fox,E Castanha,A Fox,C Feigley,D Salzberg,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of environmental monitoring : JEM

VOLUME: 10

Page Numbers: 55-9

Journal Abbreviation:

ISSN: 1464-0325

DAY: 9

MONTH: 11

YEAR: 2007

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100968688

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms.

AFFILIATION: Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, SC 29208, Columbia, USA. afox@med.sc.edu.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Environ Monit

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms Related Publications

• A computational survey of candidate exonic splicing enhancer motifs in the model plant Arabidopsis thaliana.
• A mechanical spike accompanies the action potential in Mammalian nerve terminals.
• A unified model explaining the offsets of overlapping and near-overlapping prokaryotic genes.
• Additional sex combs affects antennal development by means of spatially restricted repression of Antp and wg.
• Bioinformatics challenges of new sequencing technology.
• Hawkeye: an interactive visual analytics tool for genome assemblies.
• Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms.
• Identifying bacterial genes and endosymbiont DNA with Glimmer.
• Is RAS Blockade Routinely Indicated in Hypertensive Kidney Transplant Patients?
• It is time to end the patenting of software.
• JIGSAW, GeneZilla, and GlimmerHMM: puzzling out the features of human genes in the ENCODE regions.
• Posterior tuberculous sinuses of vertebral origin; wound revision and closure with streptomycin permitting early spinal fusion.
• Rapid, accurate, computational discovery of Rho-independent transcription terminators illuminates their relationship to DNA uptake.
• Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18.
• Subcutaneous mastectomy and immediate reconstruction for prevention of breast cancer for high-risk patients.
• The contents of the syringe.
• The neurologist, psychogenic nonepileptic seizures, and borderline personality disorder.
• The use of streptomycin in thoracoplasty for pulmonary tuberculosis.
• Transtracheal anesthesia for bronchoscopy.
• Two-photon excitation of potentiometric probes enables optical recording of action potentials from Mammalian nerve terminals in situ.
• Using GlimmerM to Find Genes in Eukaryotic Genomes.
• [Humana banana cereal in daily practice]
• [Treatment of acute diarrhea in pediatrics with Streptococcus faecium: results of a double blind study]