Human embryo development and pregnancies in an homologous granulosa cell coculture system.

Human embryo development and pregnancies in an homologous granulosa cell coculture system. Research Abstract Details 

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Human embryo development and pregnancies in an homologous granulosa cell coculture system. Abstract Text:

PURPOSE: Our purpose was to determine the effects of the coculture of embryos on human granulosa cells (GCs) in patients in the first cycle of IVF-ET treatment and in patients with repeated implantation failures and to investigate the presence of specific proteins in a 48-hr GC conditioned medium and the GC ultrastructural characteristics. METHODS: Eighteen patients with tubal or idiopathic infertility were enrolled in this study: 7 patients (Trial 1) were in the first cycle of IVF-ET treatment and 11 patients (Trial 2) had repeated implantation failures (one to five). Embryos from each patient were cocultured randomly either on homologous granulosa cells or on a conventional culture medium. RESULTS: At the end of the coculture period (day 5 or 6), 50% of the embryos (Trial 1) reached the blastocyst stage, with respect to 35% in Trial 2. The pregnancy rate per retrieval was 14.2 and 9%, respectively, in Trial 1 and in Trial 2. Many conditioned media showed proteins of 24-29 kDa. and some of them showed additional proteins of 90 kDa. The ultrastructural analysis of GCs showed healthy, metabolically active, protein-synthesizing, and mostly steroidogenic cells. CONCLUSIONS: GC cultures improve embryo development but not pregnancy rates both in Trial 1 and in Trial 2.

Human embryo development and pregnancies in an homologous granulosa cell coculture system. Publishing Authors By Initials

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Human embryo development and pregnancies in an homologous granulosa cell coculture system. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 17

Page Numbers: 1-12

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Jan

YEAR: 2000

Human embryo development and pregnancies in an homologous granulosa cell coculture system. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Human embryo development and pregnancies in an homologous granulosa cell coculture system. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: secretion

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Substance Name: Proteins

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Grant and Affiliation Information for Human embryo development and pregnancies in an homologous granulosa cell coculture system.

AFFILIATION: Infertility and IVF Centre, University of Bologna, Italy.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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