Human corneal stem cells display functional neuronal properties.

Human corneal stem cells display functional neuronal properties. Research Abstract Details 

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Human corneal stem cells display functional neuronal properties. Abstract Text:

PURPOSE: Human corneal limbal stem cells mature and repopulate the superficial layers of the cornea throughout life. In this study, we tested the hypothesis that human corneal stem cells, derived from neural ectoderm, can exhibit functional neuronal properties. METHODS: Human corneal limbal tissue (donor age 6 weeks to 92 years) was obtained from Upstate New York Transplant Services. Tissues were grown as explants on coverslips in DMEM with 10% calf serum. After 7-14 days in vitro, tissues on coverslips were double-immunostained for the stem cell marker, p63, along with nestin and neurotransmitter receptors GABA, dopamine, serotonin, glycine or acetylcholine. We also carried out whole cell current clamp and voltage clamp recordings on corneal stem cell cultures in order to determine their functional neurophysiological properties. RESULTS: Co-localization of p63 with nestin, GABA receptor, glycine receptor, and serotonin receptor immunoreactivity was seen in a small number of cells in the corneal stem cell cultures. The resting potential of corneal stem cells was relatively low, approximately -13+/-8 mV (n=13; range -6 mV to -40 mV) measured in current clamp. No action potentials or voltage sensitive Na+ and K+ currents were detected. However, in a small number of cells, kainic acid (0.5 mM), a non-NMDA glutamate receptor agonist, and GABA induced a small inward current. Glutamate receptor antagonist, CNQX, and GABA receptor antagonist, bicuculline and CGP-35348 blocked the agonist response. CONCLUSIONS: A subpopulation of human corneal stem cells exhibit neuronal properties in vitro, as evidenced by immunoreactivity to nestin, GABA receptor, glycine receptor, and serotonin receptor, as well as functional neurophysiological responses to GABA and kainic acid. Human corneal stem cells may represent a potential source of non-embryonic, autologous, surgically-accessible graft material with neuronal potential.

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Human corneal stem cells display functional neuronal properties. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular vision

VOLUME: 9

Page Numbers: 159-63

Journal Abbreviation: Mol. Vis.

ISSN: 1090-0535

DAY: 30

MONTH: 04

YEAR: 2003

Human corneal stem cells display functional neuronal properties. Information

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LANGUAGE: eng

NlmUniqueID: 9605351

Human corneal stem cells display functional neuronal properties. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Human corneal stem cells display functional neuronal properties. Information

Substance Name: nestin

Registry Number: 0

Grant and Affiliation Information for Human corneal stem cells display functional neuronal properties.

AFFILIATION: Department of Ophthalmology, University at Buffalo, Buffalo, NY 14214, USA. gseigel@frontiernet.net

Country: United States

AGENCY: United States NIDCD

GRANT: P01 DC03600-01A1

ACRONYM: DC

MEDLINETA: Mol Vis

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