Human ACF1 alters the remodeling strategy of SNF2h.

Human ACF1 alters the remodeling strategy of SNF2h. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Human ACF1 alters the remodeling strategy of SNF2h. Abstract Text:

Xi He,Hua-Ying Fan,Geeta J Narlikar,Robert E Kingston,

The human ACF chromatin-remodeling complex (hACF) contains the ATPase motor protein SNF2h and the non-catalytic hACF1 subunit. Here, we have compared the ability of SNF2h and a reconstituted hACF complex containing both SNF2h and hACF1 to remodel a series of nucleosomes containing different lengths of DNA overhang. Both SNF2h and hACF functioned in a manner consistent with sliding a canonical nucleosome. However, the non-catalytic subunit, hACF1, altered the remodeling properties of SNF2h by changing the nature of the requirement for a DNA overhang in the nucleosomal substrate and altering the DNA accessibility profile of the remodeled products. Surprisingly, addition of hACF1 to SNF2h increased the amount of DNA overhang needed to observe measurable amounts of DNA accessibility, but decreased the amount of overhang needed for a measurable binding interaction. We propose that these hACF1 functions might contribute to making the hACF complex more efficient at nucleosome spacing compared with SNF2h. In contrast, the SWI/SNF complex and its ATPase subunit BRG1 generated DNA accessibility profiles that were similar to each other, but different significantly from those of hACF and SNF2h. Thus, we observed divergent remodeling behaviors in these two remodeling families and found that the manner in which hACF1 alters the remodeling behavior of the ATPase is not shared by SWI/SNF subunits.

Human ACF1 alters the remodeling strategy of SNF2h. Publishing Authors By Initials

X He,HY Fan,GJ Narlikar,RE Kingston,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Human ACF1 alters the remodeling strategy of SNF2h. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 28636-47

Journal Abbreviation:

ISSN: 0021-9258

DAY: 29

MONTH: 07

YEAR: 2006

Human ACF1 alters the remodeling strategy of SNF2h. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Human ACF1 alters the remodeling strategy of SNF2h. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Human ACF1 alters the remodeling strategy of SNF2h. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Human ACF1 alters the remodeling strategy of SNF2h.

AFFILIATION: Department of Molecular Biology, Massachusetts General Hospital, Boston, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Human ACF1 alters the remodeling strategy of SNF2h Related Publications

• A clinical trial of an antidepressant, tranylcy-promine ("Parnate").
• A not so Pacific voyage: the 'floating laboratory' of Nicolas Baudin.
• Antiplasmodial activity of compounds from Sloanea rhodantha (Baker) Capuron var. rhodantha from the Madagascar rain forest.
• Antiproliferative Cassane Diterpenoids of Cordyla madagascariensis ssp. madagascariensis from the Madagascar Rainforest(1).
• Antiproliferative cardenolides of an Elaeodendron sp. from the Madagascar rain forest(1).
• Antiproliferative prenylated stilbenes and flavonoids from Macaranga alnifolia from the Madagascar rainforest.
• Antiproliferative xanthones of Terminalia calcicola from the Madagascar rain forest.
• Bridging converts a noncytotoxic nor-paclitaxel derivative to a cytotoxic analogue by constraining it to the T-Taxol conformation.
• Cytotoxic Cardenolide Glycosides of Roupellina (Strophanthus) boivinii from the Madagascar Rainforest.
• Cytotoxic clerodane diterpenoids and their hydrolysis products from Casearia nigrescens from the rainforest of Madagascar.
• Cytotoxic compounds of Physena madagascariensis from the Madagascar rain forest paragraph sign.
• Cytotoxic compounds of Schizolaena hystrix from the Madagascar rainforest.
• Cytotoxic triterpenoid saponins of Albizia gummifera from the Madagascar rain forest.
• DNase I Footprint Analysis of Protein-DNA Binding.
• Direct analysis of RNA after transfection.
• Effectiveness of chiropractic treatment for infantile colic.
• Evaluation of the tubulin-bound paclitaxel conformation: synthesis, biology, and SAR studies of C-4 to C-3' bridged paclitaxel analogues.
• Fund raising: innovative fund raising brings art to a hospital.
• Furoquinoline alkaloids of Ertela (Monnieria) trifolia (L.) Kuntze from the Suriname rainforest.
• Gene regulation in the postgenomic era: technology takes the wheel.
• Guttiferones K and L, antiproliferative compounds of Rheedia calcicola from the Madagascar rain forest.
• Homology-driven chromatin remodeling by human RAD54.
• Human ACF1 alters the remodeling strategy of SNF2h.
• Independent and complementary methods for large-scale structural analysis of mammalian chromatin.
• Ipomoeassin F, a new cytotoxic macrocyclic glycoresin from the leaves of Ipomoea squamosa from the Suriname rainforest.
• Preparation of poly(a)(+) RNA.
• Rotational-echo double-resonance NMR distance measurements for the tubulin-bound Paclitaxel conformation.
• Shifting adaptive landscapes: progress and challenges in reconstructing early hominid environments.
• Tambouranolide, a new cytotoxic hydroxybutanolide from a Tambourissa sp. (Monimiaceae).
• The shape of things to come: structural and synthetic studies of taxol and related compounds.