Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 vpx-mediated preintegration complex.

Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 vpx-mediated preintegration complex. Research Abstract Details 

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Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 vpx-mediated preintegration complex. Abstract Text:

Xiaogang Cheng,Michael Belshan,Lee Ratner,Xiaogang Cheng,Michael Belshan,Lee Ratner,Xiaogang Cheng,Michael Belshan,Lee Ratner,

Human immunodeficiency virus type 2 (HIV-2) Vpx is required for nuclear translocation of the viral preintegration complex (PIC) in quiescent cells. In order to decipher the mechanism of action of Vpx, a cDNA library was screened with the yeast two-hybrid assay, resulting in the identification of heat shock protein 40, Hsp40/DnaJB6, as a Vpx-interactive protein. Interaction with Vpx was confirmed by glutathione S-transferase (GST) pull-down and coimmunoprecipitation assays. Overexpression of Hsp40/DnaJB6 enhanced Vpx nuclear import, whereas overexpression of a nuclear localization mutant of Hsp40/DnaJB6 (H31Q) or down-regulation of Hsp40/DnaJB6 by small interfering RNA (siRNA) reduced the nuclear import of Vpx. Hsp40/DnaJB6 competed with the Pr55(Gag) precursor protein for the binding of Vpx and incorporation into virus-like particles. Overexpression of Hsp40/DnaJB6 promoted viral PIC nuclear import, whereas siRNA down-regulation of Hsp40/DnaJB6 inhibited PIC nuclear import. These results demonstrate a role for Hsp40/DnaJB6 in the regulation of HIV-2 PIC nuclear transport.

Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 vpx-mediated preintegration complex. Publishing Authors By Initials

X Cheng,M Belshan,L Ratner,X Cheng,M Belshan,L Ratner,X Cheng,M Belshan,L Ratner,

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Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 vpx-mediated preintegration complex. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of virology

VOLUME: 82

Page Numbers: 1229-37

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 21

MONTH: 11

YEAR: 2007

Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 vpx-mediated preintegration complex. Information

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LANGUAGE: eng

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AFFILIATION: Box 8069, 660 S. Euclid Ave., Washington University School of Medicine, St. Louis, MO 63110. lratner@im.wustl.edu.

Country: United States

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MEDLINETA: J Virol

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