HPLC determinations of enantiomeric ratios.

HPLC determinations of enantiomeric ratios. Research Abstract Details 

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HPLC determinations of enantiomeric ratios. Abstract Text:

Peter Wipf,Stefan Werner,Leslie A Twining,Christopher Kendall,

Evaporative light-scattering detection (ELSD) is widely applied in the HPLC analysis of organic compounds lacking a UV chromophore. However, this detection method is generally unsuitable for determination of enantiomeric ratios (er). The er calculated from a UV trace and an ELS trace of the same compound differs significantly. Because of the nonlinear concentration response of the ELS detector, a compound with an er of 95:5 appears to be enantiomerically pure by ELS detection. It is possible to obtain a calibration curve and to calculate a correction factor, but this procedure is time consuming and therefore not very practical for routine analyses. In contrast, a charged aerosol detector allows a very accurate determination of the enantiomeric ratios. Like the ELS detection, the CA detection is independent of the chromophore properties of the substrate. Therefore, we recommend the use of CA instead of ELS detection for determination of the enantiomeric ratios of non-UV active compounds.

HPLC determinations of enantiomeric ratios. Publishing Authors By Initials

P Wipf,S Werner,LA Twining,C Kendall,

For similar natural sciences: chemistry: chemistry, organic: isomerism: stereoisomerism research abstracts see: natural sciences: chemistry: chemistry, organic: isomerism: stereoisomerism research

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HPLC determinations of enantiomeric ratios. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Chirality

VOLUME: 19

Page Numbers: 5-9

Journal Abbreviation:

ISSN: 0899-0042

DAY: 3

MONTH: Jan

YEAR: 2007

HPLC determinations of enantiomeric ratios. Information

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LANGUAGE: eng

NlmUniqueID: 8914261

HPLC determinations of enantiomeric ratios. Keywords Mesh Terms:

KEYWORDS: Stereoisomerism

MESH TERMS: methods

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Grant and Affiliation Information for HPLC determinations of enantiomeric ratios.

AFFILIATION: Center for Chemical Methodologies and Library Development (UPCMLD), University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA. pwipf@pitt.edu

Country: United States

AGENCY: United States NIGMS

GRANT: P50-GM067082

ACRONYM: GM

MEDLINETA: Chirality

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