[HPLC analysis of microcystin-RR, YR and LR from Dianshan Lake]

[HPLC analysis of microcystin-RR, YR and LR from Dianshan Lake] Research Abstract Details 

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[HPLC analysis of microcystin-RR, YR and LR from Dianshan Lake] Abstract Text:

Li-hong Yin,Guo-zhou Zhang,Guo-guang Cheng,Si-shun Wei,

OBJECTIVE: To optimize the solid-phase extraction method of isolation, purification and determination of intracellular microcystin-RR, -YR and -LR (MC-RR,-YR,-LR), algal samples were collected from Dianshan Lake, which is a major water source of Shanghai. METHODS: During extraction, Algal samples were treated with different solvent (5% acetic acid and 75% methanol), at different temperature (room temperature and 56 degrees C), centrifuge at 10000 x g, 30 min and 3500 rpm/min, 10 min, eluted with 80% methanol with or without trifluoroacetate acid(TFA). Further, gradient elution were employed to separate microcystins in high performance liquid chromatography (HPLC), the mobile phase consisted of aqueous acetonitrile and 0.05% TFA. RESULTS: When dried algae cells were extracted by 5% acetic acid, 56 degrees C water bath combined with centrifuge at 3500 rpm/min was an effective and simple method for the extraction of microcystin-RR, elutant contains TFA can markedly increase the yield of microcystins extraction; the concentration of microcystin-RR in nature algal samples was higher than MC-LR and MC-YR. CONCLUSION: The extraction efficacy of MC-YR and -LR is higher in 75% methanol aqueous and eluted with 80% methanol containing 0.05% TFA, while the extraction efficacy of MC-RR is higher in 5% acetic acid.

[HPLC analysis of microcystin-RR, YR and LR from Dianshan Lake] Publishing Authors By Initials

LH Yin,GZ Zhang,GG Cheng,SS Wei,

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[HPLC analysis of microcystin-RR, YR and LR from Dianshan Lake] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Wei sheng yan jiu = Journal of hygiene research

VOLUME: 35

Page Numbers: 162-4

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ISSN: 1000-8020

DAY: 8

MONTH: Mar

YEAR: 2006

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LANGUAGE: chi

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AFFILIATION: Key Laboratory of the Public Health and Safety, Ministry of Education, Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China.

Country: China

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MEDLINETA: Wei Sheng Yan Jiu

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