Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung.

Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Research Abstract Details 

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Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Abstract Text:

MaryAnn V Volpe,Sujatha M Ramadurai,Lucia D Pham,Heber C Nielsen,

Organ-specific patterning is partly determined by Hox gene regulatory interactions with the extracellular matrix (ECM), cell adhesion and fibroblast growth factor (FGFs) signaling pathways but coordination of these mechanisms in lung development is unknown. We have previously shown that Hoxb-5 affects airway patterning during lung morphogenesis. Hoxb-5 regulation in fetal lung affects ECM expression of tenascin-C and alters FGF10 spatial and cellular expression. To test this hypothesis, gestational day 13.5 (Gd13.5) fetal mouse lung fibroblasts and whole lungs were cultured with Hoxb-5-specific small interfering RNA (siRNA). Western blots showed that siRNA-down regulation of Hoxb-5 led to decreased tenascin-C and FGF10 and was associated with increased Hoxb-4 and decreased Hoxb-6 protein levels. Hoxa-5 protein levels were not affected. Hoxb-5 siRNA-treated whole lung cultures had a significant decrease in total lung and peripheral branching region surface area. Immunostaining showed negligible levels of Hoxb-5 protein and tenascin-C, and loss of FGF10 spatial restriction. We conclude that Hoxb-5 helps regulate lung airway development through modulation of ECM expression of tenascin-C. ECM changes induced by Hoxb-5 may affect mesenchymal-epithelial cell signaling to alter spatial and cellular restriction of FGF10. Hoxb-5 may also affect lung airway branching indirectly by cross regulation of other Hoxb genes.

Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Publishing Authors By Initials

MV Volpe,SM Ramadurai,LD Pham,HC Nielsen,

For similar proteins: scleroproteins: extracellular matrix proteins: tenascin research abstracts see: proteins: scleroproteins: extracellular matrix proteins: tenascin research

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Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Frontiers in bioscience : a journal and virtual li

VOLUME: 12

Page Numbers: 860-73

Journal Abbreviation: Front. Biosci.

ISSN: 1093-4715

DAY: 1

MONTH: 01

YEAR: 2007

Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9709506

Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Keywords Mesh Terms:

KEYWORDS: Tenascin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung. Information

Substance Name: Tenascin

Registry Number: 0

Grant and Affiliation Information for Hoxb-5 down regulation alters Tenascin-C, FGF10 and Hoxb gene expression patterns in pseudoglandular period fetal mouse lung.

AFFILIATION: Department of Pediatrics, Division of Newborn Medicine, Tufts-New England Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA. mvolpe1@tufts-nemc.org

Country: United States

AGENCY: United States NHLBI

GRANT: HL37930

ACRONYM: HL

MEDLINETA: Front Biosci

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