How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS).

How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Research Abstract Details 

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How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Abstract Text:

Yuming Xiao,Karl Fisher,Matt C Smith,William E Newton,David A Case,Simon J George,Hongxin Wang,Wolfgang Sturhahn,Ercan E Alp,Jiyong Zhao,Yoshitaka Yoda,Stephen P Cramer,

Nitrogenase catalyzes a reaction critical for life, the reduction of N(2) to 2NH(3), yet we still know relatively little about its catalytic mechanism. We have used the synchrotron technique of (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to study the dynamics of the Fe-S clusters in this enzyme. The catalytic site FeMo-cofactor exhibits a strong signal near 190 cm(-)(1), where conventional Fe-S clusters have weak NRVS. This intensity is ascribed to cluster breathing modes whose frequency is raised by an interstitial atom. A variety of Fe-S stretching modes are also observed between 250 and 400 cm(-)(1). This work is the first spectroscopic information about the vibrational modes of the intact nitrogenase FeMo-cofactor and P-cluster.

How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Publishing Authors By Initials

Y Xiao,K Fisher,MC Smith,WE Newton,DA Case,SJ George,H Wang,W Sturhahn,EE Alp,J Zhao,Y Yoda,SP Cramer,

For similar biomechanics: vibration research abstracts see: biomechanics: vibration research

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How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 7608-12

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 14

MONTH: Jun

YEAR: 2006

How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Keywords Mesh Terms:

KEYWORDS: Vibration

MESH TERMS: chemistry

Chemical & Substance for Abstract: How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). Information

Substance Name: Nitrogenase

Registry Number: EC 1.18.6.1

Grant and Affiliation Information for How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS).

AFFILIATION: Department of Applied Science, University of California, Davis, California 95616, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-65440

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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