How metal ions affect amyloid formation: Cu2+- and Zn2+-sensitive peptides.

How metal ions affect amyloid formation: Cu2+- and Zn2+-sensitive peptides. Research Abstract Details 

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How metal ions affect amyloid formation: Cu2+- and Zn2+-sensitive peptides. Abstract Text:

The common feature of proteins involved in many neurodegenerative diseases is their ability to adopt at least two different stable conformations. The conformational transition that shifts the equilibrium from the functional, mostly partially alpha-helical structure, to the beta-sheet rich amyloid can be triggered by numerous factors, such as mutations in the primary structure or changes in the environment. We present a set of model peptides that, without changes in their primary structure, react in a predictable fashion in the presence of transition metal ions by adopting different conformations and aggregate morphologies. These de novo designed peptides strictly follow the characteristic heptad repeat of the alpha-helical coiled-coil structural motif. Furthermore, domains that favor beta-sheet formation have been incorporated to make the system prone to amyloid formation. As a third feature, histidine residues create sensitivity towards the presence of transition metal ions. CD spectroscopy, ThT fluorescence experiments, and transmission electron microscopy were used to characterize peptide conformation and aggregate morphology in the presence of Cu2+ and Zn2+. Furthermore, the binding geometry within peptide-Cu2+ complexes was characterized by electron paramagnetic resonance spectroscopy.

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How metal ions affect amyloid formation: Cu2+- and Zn2+-sensitive peptides. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chembiochem : a European journal of chemical biolo

VOLUME: 9

Page Numbers: 531-6

Journal Abbreviation: Chembiochem

ISSN: 1439-7633

DAY: 3

MONTH: Mar

YEAR: 2008

How metal ions affect amyloid formation: Cu2+- and Zn2+-sensitive peptides. Information

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LANGUAGE: eng

NlmUniqueID: 100937360

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Grant and Affiliation Information for How metal ions affect amyloid formation: Cu2+- and Zn2+-sensitive peptides.

AFFILIATION: Institute for Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.

Country: Germany

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MEDLINETA: Chembiochem

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