Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences.

Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Research Abstract Details 

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Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Abstract Text:

A collection of yeast strains bearing single marked Ty1 insertions on chromosome III was generated. Over 100 such insertions were physically mapped by pulsed-field gel electrophoresis. These insertions are very nonrandomly distributed. Thirty-two such insertions were cloned by the inverted PCR technique, and the flanking DNA sequences were determined. The sequenced insertions all fell within a few very limited regions of chromosome III. Most of these regions contained tRNA coding regions and/or LTRs of preexisting transposable elements. Open reading frames were disrupted at a far lower frequency than expected for random transposition. The results suggest that the Ty1 integration machinery can detect regions of the genome that may represent "safe havens" for insertion. These regions of the genome do not contain any special DNA sequences, nor do they behave as particularly good targets for Ty1 integration in vitro, suggesting that the targeted regions have special properties allowing specific recognition in vivo.

Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Publishing Authors By Initials

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Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Cell

VOLUME: 73

Page Numbers: 1007-18

Journal Abbreviation: Cell

ISSN: 0092-8674

DAY: 4

MONTH: Jun

YEAR: 1993

Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Information

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LANGUAGE: eng

NlmUniqueID: 413066

Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae

MESH TERMS: genetics

Chemical & Substance for Abstract: Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences. Information

Substance Name: GCGGCCGC-specific type II deoxyribonucle

Registry Number: EC 3.1.21.4

Grant and Affiliation Information for Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences.

AFFILIATION: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: T32GM07445

ACRONYM: GM

MEDLINETA: Cell

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