Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release.

Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release. Research Abstract Details 

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Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release. Abstract Text:

Dennis K Galanakis,Marguerite Neerman-Arbez,Tomas Scheiner,Agnes Henschen,Doris Hubbs,Chandrasekaran Nagaswami,John W Weisel,Dennis K Galanakis,Marguerite Neerman-Arbez,Tomas Scheiner,Agnes Henschen,Doris Hubbs,Chandrasekaran Nagaswami,John W Weisel,Dennis K Galanakis,Marguerite Neerman-Arbez,Tomas Scheiner,Agnes Henschen,Doris Hubbs,Chandrasekaran Nagaswami,John W Weisel,

We detail for the first time the uniquely altered fibrin polymerization of homophenotypic Aalpha R16H dysfibrinogen. By polymerase chain reaction amplification and DNA sequencing, our new proposita's genotype consisted of a G>A transition encoding for Aalpha R16H, and an 11 kb Aalpha gene deletion. High-performance liquid chromatography disclosed fibrinopeptide A release approximately six times slower than its fibrinopeptide B. Turbidimetric analyses revealed unimpaired fibrin repolymerization, and abnormal thrombin-induced polymerization (1-7 mumol/l fibrinogen, > 96% coagulable), consisting of a prolonged lag time, slow rate, and abnormal clot turbidity maxima, all varying with thrombin concentration. For example, at 0.2-3 U/ml, the resulting turbidity maxima ranged from lower to higher than normal control values. By scanning electron microscopy, clots formed by 0.3 and 3 thrombin U/ml displayed mean fibril diameters 42 and 254% of the respective control values (n = 400). Virtually no such differences from control values were demonstrable, however, when clots formed in the presence of high ionic strength (mu = 0.30) or of monoclonal antibeta(15-42)IgG. The latter also prolonged the thrombin clotting time approximately three-fold. Additionally, thrombin-induced clots displayed decreased elastic moduli, with G' values of clots induced by 0.3, 0.7 and 3 thrombin U/ml corresponding to 11, 34, and 45% of control values. The results are consistent with increased des-BB fibrin monomer generation preceding and during polymerization. This limited the inherent gelation delay, decreased the clot stiffness, and enabled a progressively coarser, rather than finer, network induced by increasing thrombin concentrations. We hypothesize that during normal polymerization these constitutive des-BB fibrin monomer properties attenuate their des-AA fibrin counterparts.

Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release. Publishing Authors By Initials

DK Galanakis,M Neerman-Arbez,T Scheiner,A Henschen,D Hubbs,C Nagaswami,JW Weisel,DK Galanakis,M Neerman-Arbez,T Scheiner,A Henschen,D Hubbs,C Nagaswami,JW Weisel,DK Galanakis,M Neerman-Arbez,T Scheiner,A Henschen,D Hubbs,C Nagaswami,JW Weisel,

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Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Blood coagulation & fibrinolysis : an internationa

VOLUME: 18

Page Numbers: 731-7

Journal Abbreviation: Blood Coagul. Fibrinolysis

ISSN: 0957-5235

DAY: 5

MONTH: Dec

YEAR: 2007

Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release. Information

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LANGUAGE: eng

NlmUniqueID: 9102551

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AFFILIATION: aSUNY, Stony Brook, New York, USA bUniversity Medical Centre, Geneva, Switzerland cUniversity of Pennsylvania, Philadelphia, Pennsylvania, USA dUCI, Irvine, California, USA eEast Tennessee State University, Johnson City, Tennessee, USA.

Country: England

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MEDLINETA: Blood Coagul Fibrinolysis

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