Homology-driven chromatin remodeling by human RAD54.

Homology-driven chromatin remodeling by human RAD54. Research Abstract Details 

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Homology-driven chromatin remodeling by human RAD54. Abstract Text:

Zhaoqing Zhang,Hua-Ying Fan,Joseph A Goldman,Robert E Kingston,

Human RAD51 and RAD54 are key players in homologous recombination, a process that requires homology recognition and strand invasion by a RAD51-single-stranded DNA (ssDNA) nucleoprotein filament and chromatin remodeling by RAD54. Here we use in vitro chromatin reconstitution systems to show that RAD51-ssDNA stimulates RAD54-dependent chromatin remodeling in a homology-dependent, polarity-independent manner. This stimulation was not seen with RAD54B or other remodelers. Chromatin remodeling by RAD54 enabled strand invasion by RAD51-ssDNA on nucleosomal templates, which was homology- and polarity-dependent. Three natural RAD54 mutants found in primary cancer cells showed specific defects in remodeling or in the RAD54-RAD51 interaction. We propose that RAD54 is recruited by RAD51-ssDNA filament to the chromatin of the intact chromosome and that it remodels that chromatin to facilitate accessibility for strand exchange.

Homology-driven chromatin remodeling by human RAD54. Publishing Authors By Initials

Z Zhang,HY Fan,JA Goldman,RE Kingston,

For similar enzymes and coenzymes: enzymes: recombinases: rec a recombinases: rad51 recombinase research abstracts see: enzymes and coenzymes: enzymes: recombinases: rec a recombinases: rad51 recombinase research

PUBMED ID PMID:

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Homology-driven chromatin remodeling by human RAD54. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature structural & molecular biology

VOLUME: 14

Page Numbers: 397-405

Journal Abbreviation:

ISSN: 1545-9993

DAY: 8

MONTH: 04

YEAR: 2007

Homology-driven chromatin remodeling by human RAD54. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101186374

Homology-driven chromatin remodeling by human RAD54. Keywords Mesh Terms:

KEYWORDS: Rad51 Recombinase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Homology-driven chromatin remodeling by human RAD54. Information

Substance Name: Rad51 Recombinase

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Homology-driven chromatin remodeling by human RAD54.

AFFILIATION: Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM48405

ACRONYM: GM

MEDLINETA: Nat Struct Mol Biol

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