Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI.

Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Research Abstract Details 

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Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Abstract Text:

Kevin D Corbett,Piero Benedetti,James M Berger,

Type II topoisomerases help disentangle chromosomes to facilitate cell division. To advance understanding of the structure and dynamics of these essential enzymes, we have determined the crystal structure of an archaeal type IIB topoisomerase, topo VI, at 4.0-A resolution. The 220-kDa heterotetramer adopts a 'twin-gate' architecture, in which a pair of ATPase domains at one end of the enzyme is poised to coordinate DNA movements into the enzyme and through a set of DNA-cleaving domains at the other end. Small-angle X-ray scattering studies show that nucleotide binding elicits a major structural reorganization that is propagated to the enzyme's DNA-cleavage center, explaining how ATP is coupled to DNA capture and strand scission. These data afford important insights into the mechanisms of topo VI and related proteins, including type IIA topoisomerases and the Spo11 meiotic recombination endonuclease.

Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Publishing Authors By Initials

KD Corbett,P Benedetti,JM Berger,

For similar archaea: crenarchaeota: sulfolobales: sulfolobaceae: sulfolobus research abstracts see: archaea: crenarchaeota: sulfolobales: sulfolobaceae: sulfolobus research

PUBMED ID PMID:

MEDLINE DATE:

Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature structural & molecular biology

VOLUME: 14

Page Numbers: 611-9

Journal Abbreviation: Nat. Struct. Mol. Biol.

ISSN: 1545-9993

DAY: 1

MONTH: 07

YEAR: 2007

Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101186374

Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Keywords Mesh Terms:

KEYWORDS: Sulfolobus

MESH TERMS: enzymology

Chemical & Substance for Abstract: Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Information

Substance Name: DNA topoisomerase VI

Registry Number: EC 5.99.1.-

Grant and Affiliation Information for Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI.

AFFILIATION: Department of Molecular and Cellular Biology, QB3 Institute, Stanley Hall #3220, University of California, Berkeley, California 94720-3220, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA077373

ACRONYM: CA

MEDLINETA: Nat Struct Mol Biol

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