HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene.

HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Research Abstract Details 

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HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Abstract Text:

Sudhir Jain,Yanna Li,Sai Patil,Ashok Kumar,

Angiotensinogen (AGT) is the precursor of one of the most important vasoactive hormone angiotensin II and this gene locus is associated with human essential hypertension. AGT is an acute phase protein and its gene expression is regulated by IL-6. Previous studies have identified three potential STAT-3 binding sites (APREs) located between -160 and -280 of the hAGT gene promoter but only APRE-1 (located between -271 and -279) was shown to be a bonafide enhancer for IL-6-induced promoter activity. We show here that APRE-2, located between -236 and -247, is indeed an HNF-1alpha-binding site and plays an important role in basal and IL-6 induced promoter activity of this gene. Our chromatin immunoprecipitation (ChIP) assay shows that HNF-1alpha binds to this region of the hAGT gene promoter and its recruitment is increased in the presence of IL-6 in Hep3B cells. We also show that the promoter activity of a deletion construct containing only 223 bp of the hAGT gene promoter (that contains only APRE-3) is increased after IL-6 treatment. Our ChIP assay shows that IL-6 treatment recruits STAT-3 to APRE-3 and suggests that this is also an IL6 responsive element. We have previously shown that GR binds to the proximal promoter of the hAGT gene. Since GR physically interacts with STAT-3, we propose that transcription factors GR, STAT-3, and HNF-1alpha that bind to the nucleotide sequence located between -160 and -280 of the hAGT gene promoter are responsible for IL-6 induced promoter activity of this gene.

HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Publishing Authors By Initials

S Jain,Y Li,S Patil,A Kumar,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Cell physiology

VOLUME: 293

Page Numbers: C401-10

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 2

MONTH: 05

YEAR: 2007

HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: drug effects

Chemical & Substance for Abstract: HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene. Information

Substance Name: Angiotensinogen

Registry Number: 11002-13-4

Grant and Affiliation Information for HNF-1alpha plays an important role in IL-6-induced expression of the human angiotensinogen gene.

AFFILIATION: Pathology Dept., New York Medical College, Valhalla, NY 10595, USA. sudhir_jain@nymc.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL-59547

ACRONYM: HL

MEDLINETA: Am J Physiol Cell Physiol

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